Purification and Characterization of Cyclohexanone 1,2-Monooxygenase from Exophiala jeanselmei strain KUFI-6N
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概要
- 論文の詳細を見る
Baeyer-Villiger cyclohexanone 1,2-monooxygenase (CHMO) was purified 17.1-fold from cell extracts of the fungus Exophiala jeanselmei grown on cyclohexanol to electrophoretically homogeneity by serial chromatographies. The molecular mass of the native enzyme was approximately 74 kDa by gel filtration and SDS-PAGE. Some enzymic characterizations were studied. The NH_2-terminal amino acid residues were Ala-Lys-Ser-Leu-Asp-Val-Leu-Ile-Val-Gly-Ala-Gly-Phe-Gly-Gly-Ile-Tyr-Gln-Leu-, with similarity to the bacterial CHMOs of FAD-binding and NADPH-dependent type Baeyer-Villiger monooxygenases.
- 社団法人日本農芸化学会の論文
- 2000-12-23
著者
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Nakai Yuka
Department Of Biotechnology Faculty Of Engineering And High Technology Research Center Kansai Univer
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HASEGAWA YOSHIE
Department of Biotechnology, Faculty of Engineering, Kansai University
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TOKUYAMA TAI
Department of Biotechnology, Faculty of Engineering, Kansai University
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Tokuyama T
Department Of Biotechnology Faculty Of Engineering And High Technology Research Center Kansai Univer
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Tokuyama Tai
Department Of Biotechnology Faculty Of Engineering Kansai University
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Tokuyama Tai
Faculty Of Engineering Kansai University
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Hasegawa Yoshie
Department Of Biotechnology Faculty Of Engineering Kansai University
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Hasegawa Y
Department Of Biotechnology Faculty Of Engineering Kansai University
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IWAKI Hiroaki
Department of Biotechnology, Faculty of Engineering, Kansai University
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Iwaki Hiroaki
Department Of Biotechnology Faculty Of Engineering Kansai University
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