Purification and Characterization of a Novel Cyclohexylamine Oxidase from the Cyclohexylamine-Degrading Brevibacterium oxydans IH-35A

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概要

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• Cyclohexylamine oxidase (CHAO) from a cell extract of Brevibacterium grown on cyclohexylamine was purified 50.2-fold, to electrophoretic homogeneity, by serial chromatographies. The molecular mass of the native enzyme was estimated to be approximately 50 kDa by gel filtration and SDS-PAGE. The optimum pH was 7.4 and the stable pH range was 6.0 to 7.0. The enzyme was thermostable up to 30℃. The enzyme was found to be highly specific for the deamination of alicyclic monoamines such as cyclopentylamine, cycloheptylamine, and N-methylcyclohexylamine and aliphatic monoamines, such as sec-butylamine. The apparent K_m value for cyclohexylamine was 1.23 mM. The enzyme was inhibited by flavin enzyme inhibitors such as quinine and quinacrine. The N-terminal 27 amino acid residues were determined as Gly-Ser-Val-Thr-Pro-Asp-Pro-Asp-Val-Asp-Val-Ile-Ile-His-Gly-Ala-Gly-Ile-Ser-Gly-Ser-Ala-Ala-Ala-Lys-Ala-Leu-, revealing homology to conventional flavin-containing amine oxidases (EC 1.4.3.4).

• 社団法人日本生物工学会の論文
• 1999-09-25

著者

• HASEGAWA YOSHIE

  Department of Biotechnology, Faculty of Engineering, Kansai University

• TOKUYAMA TAI

  Department of Biotechnology, Faculty of Engineering, Kansai University

• Tokuyama T

  Department Of Biotechnology Faculty Of Engineering And High Technology Research Center Kansai Univer

• Tokuyama Tai

  Department Of Biotechnology Faculty Of Engineering Kansai University

• Tokuyama Tai

  Faculty Of Engineering Kansai University

• Hasegawa Yoshie

  Department Of Biotechnology Faculty Of Engineering Kansai University

• Hasegawa Y

  Department Of Biotechnology Faculty Of Engineering Kansai University

• IWAKI Hiroaki

  Department of Biotechnology, Faculty of Engineering, Kansai University

• SHIMIZU MASATAKE

  Department of Biotechnology, Faculty of Engineering and High Technology Research Center, Kansai Univ

• Iwaki Hiroaki

  Department Of Biotechnology Faculty Of Engineering Kansai University

• Shimizu Masatake

  Department Of Biotechnology Faculty Of Engineering And High Technology Research Center Kansai Univer

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