Construction of a New Shuttle Vector pNVR-1012 and the Cloning of Salicylate Hydroxylase Gene in Pseudomonas aeruginosa and Esherichia coli
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概要
- 論文の詳細を見る
A mini-plasmid, pNVR-1012 (approximately 2.7 kb), was constructed from the Pseudomonas viridiflava novel plasmid which encoded the ice-nucleation gene. This plasmid encoded an ampicillin resistance gene, and had the unique restriction sites of EcoRI, hindIII, PstI, salI and SphI. pNVR-nahG was constructed by ligation of a 3.1-kb hindIII fragment containing the salicylate hydroxylase (nahG) gene from the NAH plasmid with HindIII-digested pNVR-1012. The nahG gene was expressed in both Escherichia coli and Pseudomonas aeruginosa harboring this recombinant plasmid.
- 公益社団法人日本生物工学会の論文
- 1994-11-25
著者
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MATSUURA Kenji
Department of Surgery, Okinawa Kenritsu Hokubu Hospital
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HASEGAWA YOSHIE
Department of Biotechnology, Faculty of Engineering, Kansai University
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Tokuyama T
Department Of Biotechnology Faculty Of Engineering And High Technology Research Center Kansai Univer
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Tokuyama Tai
Department Of Biotechnology Faculty Of Engineering Kansai University
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Hasegawa Yoshie
Department Of Biotechnology Faculty Of Engineering Kansai University
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Hasegawa Y
Department Of Biotechnology Faculty Of Engineering Kansai University
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OHGAMA HIDEKI
Department of Biotechnology, Faculty of Engineering, Kansai University
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Matsuura Kenji
Department Of Biotechnology Faculty Of Engineering Kansai University
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