Cloning and Expression of the N, N-Dimethylformamidase Gene from Alcaligenes sp. Strain KUFA-1

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概要

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• N, N-Dimethylformamidase (DMFase) from Alcaligenes sp.strain KUFA-1,a bacterium that can grow on N, N-dimethylformamide (DMF) as the sole carbon and nitrogen source, catalyzes the first step of the DMF degradation. The DMFase gene dmfA1A2 was cloned in Escherichia coli, and its nucleotides were sequenced. The deduced amino acid sequence of the enzyme consisted of two α-and two β-subunits with 132 and 762 amino acids, respectively, and had little similarity to sequences in protein databases, including various amidases. The protein may be a new kind of amidase. DMFase activity was detected in E. coli cells transformed with an expression plasmid of the cloned DMFase gene. The properties of recombinant DMFase purified from E.coli were identical to those of Alcaligenes DMFase.

• 社団法人日本農芸化学会の論文
• 1999-12-23

著者

• HASEGAWA YOSHIE

  Department of Biotechnology, Faculty of Engineering, Kansai University

• TOKUYAMA TAI

  Department of Biotechnology, Faculty of Engineering, Kansai University

• Tokuyama T

  Department Of Biotechnology Faculty Of Engineering And High Technology Research Center Kansai Univer

• Tokuyama Tai

  Department Of Biotechnology Faculty Of Engineering Kansai University

• Tokuyama Tai

  Faculty Of Engineering Kansai University

• Hasegawa Yoshie

  Department Of Biotechnology Faculty Of Engineering Kansai University

• Hasegawa Y

  Department Of Biotechnology Faculty Of Engineering Kansai University

• IWAKI Hiroaki

  Department of Biotechnology, Faculty of Engineering, Kansai University

• Iwaki Hiroaki

  Department Of Biotechnology Faculty Of Engineering Kansai University

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