Expression of the Ice Nucleation Active Gene of a Novel Plasmid pNVR-1 from Pseudomonas viridiflava in Escherichia coli and Pseudomonas aeruginosa
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概要
- 論文の詳細を見る
A novel plasmid pNVR-1,a self-circularized DNA segment which consists of a 13.2 kb-EcoRI fragment from Pseudomonas viridiflava KUIN-2 total DNA, was introduced into both Escherichia coli JM109 and P. aeruginosa PAO 1161 by transformation. Both of the strains harboring the pNVR-1 plasmid showed good ice nucleation activity (INA) and resistance to ampicillin. In addition, the optimum growth temperatures for these strains shifted to temperatures lower than those of strains without pNVR-1. The pNVR-1 was more stable in E. coli cells than in P. aeruginosa cells. The number of copies of pNVR-1 in E. coli cells was approximately 20 per chromosome equivalent.
- 公益社団法人日本生物工学会の論文
- 1992-08-25
著者
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Izumi Koji
Departmcnt Of Obstetrics And Gynecology Gihoku General Hospital
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Izumi Koji
Department Of Biotechnology Faculty Of Engineering Kansai Universtiy
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HASEGAWA YOSHIE
Department of Biotechnology, Faculty of Engineering, Kansai University
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Tokuyama Tai
Department Of Biotechnology Faculty Of Engineering Kansai University
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Obata Hitoshi
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol
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Hasegawa Yoshie
Department Of Biotechnology Faculty Of Engineering Kansai University
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Hasegawa Y
Department Of Biotechnology Faculty Of Engineering Kansai University
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OHGAMA HIDEKI
Department of Biotechnology, Faculty of Engineering, Kansai University
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