Production of Two Types of Ice Crystal-Controlling Proteins in Antarctic Bacterium(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
Pseudomonas fluorescens KUAF-68, which was isolated from Antarctica, had both ice-nucleating protein and antifreeze protein activities in the culture broth. We found that both proteins were separately produced based on the results of column chromatography, SDS-PAGE analysis and Southern hybridization. The activity of the ice-nucleating protein was stimulated by the addition of glycine (0.020N%), whereas the activity of the antifreeze protein was stimulated by the addition of L-asparagine (0.025N%). This is the first report on the production of two types of ice crystal-controlling proteins in one bacterial strain.
- 社団法人日本生物工学会の論文
- 2004-09-25
著者
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OBATA Hitoshi
Graduate School of Bioresources, Mie University
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Obata H
Graduate School Of Bioresources Mie University
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Obata H
Department Of Biotechnology Faculty Of Engineering Kansai University
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Obata Hitoshi
Department Of Biotechnology Faculty Of Engineering Kansai University
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NAKANO Yoko
Department of Oral and Maxillofacial Surgery, Tokyo Dental College
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Kawahara Hidehisa
Department of Biotechnology, Faculty of Engineering, Kansai University
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MURYOI Naomi
Department of Biotechnology, Faculty of Engineering, Kansai University
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NISHIKAWA Jiro
Department of Applied Biological Science, Faculty of Science and Technology, Science University of T
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OMIYA KAZUHIRO
Department of Biotechnology, Faculty of Engineering, Kansai University
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Hidehisa Kawahara
Department Of Biotechnology Faculty Of Engineering Kansai University:high Technology Research Center
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Kawahara Hidehisa
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Nakano Yoko
Department Of Functional Diagnostic Science Osaka University Graduate School Of Medicine
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Muryoi Naomi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Obata Hitoshi
Graduate School Of Bioresources Mie Univ.
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Obata Hitoshi
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol
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Nishikawa Jiro
Departmant Of Civil And Environmental Engineering Yamanashi University
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Omiya Kazuhiro
Department Of Biotechnology Faculty Of Engineering Kansai University
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Kawaguchi H
Department Of Orthopaedic Surgery Graduate School Of Medicine The University Of Tokyo
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Nakano Yoko
Department Of Biology Graduate School Of Science Kanagawa University
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Nakano Yoko
Department Of Biotechnology Faculty Of Engineering Kansai University
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Kawaguchi Hiroshi
Department Of Orthopaedic Surgery The University Of Tokyo
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