Purification and Properties of an Ice-Nucleating Protein from an Ice-Nucleating Bacterium, Pantoea ananatis KUIN-3
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概要
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An ice-nucleating protein (INP) from the extracellular ice-nucleating matter (EIM) of Pantoea ananatis (Erwinia uredovora) KUIN-3 was purified and characterized. The EIM produced by the strain KUIN-3 was purified by ultrafiltration, sucrose density-gradient ultracentrifugation and gel filtration. The INP was purified using of column chromatography on hydroxyapatite and Superdex 200 in the nondenaturing detergent of 0.1% (w/v) Triton X-100. The purified INP was composed of one subunit of 117 kDa according to SDS-PAGE. It has become apparent that the INP was the ice-nucleating lipoglycoprotein based on the reaction of carbohydrate stain and lipid stain with the INP. It was inhibited by p-mercuribenzoate and N-bromosuccinimide. The activity of the INP gradually decreased from 65℃. The pH stability was held between pH 7.0 and pH 11.0. The INP had a lower ice-nucleating temperature below pH 6.0. It has become apparent that the INP consisted of the class C structure in the EIM based on its freezing difference spectrum in D_2O versus H_2O.
- 2003-02-25
著者
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OBATA Hitoshi
Graduate School of Bioresources, Mie University
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Obata H
Graduate School Of Bioresources Mie University
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Obata H
Department Of Biotechnology Faculty Of Engineering Kansai University
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Obata Hitoshi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Kawahara Hidehisa
Department of Biotechnology, Faculty of Engineering, Kansai University
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MURYOI Naomi
Department of Biotechnology, Faculty of Engineering, Kansai University
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Hidehisa Kawahara
Department Of Biotechnology Faculty Of Engineering Kansai University:high Technology Research Center
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Kawahara Hidehisa
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Muryoi Naomi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Obata Hitoshi
Graduate School Of Bioresources Mie Univ.
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Obata Hitoshi
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol
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MATSUKAWA KENJI
Department of Biotechnology, Faculty of Engineering, Kansai University
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YAMADE KAZUHIRO
Department of Biotechnology, Faculty of Engineering, Kansai University
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Matsukawa Kenji
Department Of Biotechnology Faculty Of Engineering Kansai University
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Yamade K
Department Of Biotechnology Faculty Of Engineering Kansai University
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Yamade Kazuhiro
Department Of Biotechnology Faculty Of Engineering Kansai University:kansai University High Technolo
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Kawaguchi H
Department Of Orthopaedic Surgery Graduate School Of Medicine The University Of Tokyo
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Kawaguchi Hiroshi
Department Of Orthopaedic Surgery The University Of Tokyo
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