Type II Antifreeze Protein from a Mid-latitude Freshwater Fish, Japanese Smelt (Hypomesus nipponensis)(Biochemistry & Molecular Biology)

概要

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A lot of reports of antifreeze protein (AFP) from fish have been published, but no report has mentioned of commercialized mid-latitude fresh water fish which producing AFP in its body fluid. We found that the AFP in the body fluid of Japanese smelt (Hypomesus nipponensis) from mid-latitude fresh water was purified and characterized. The N-terminal amino acid sequence of the Japanese smelt AFP was 75.0% identical to Type II AFP from herring. Results of EDTA treatment and ruthenium red staining suggested that the Japanese smelt AFP had at least one Ca^<2+>-binding domain. Interestingly, the antifreeze activity of the Japanese smelt AFP did not completely disappear when Ca^<2+> ions were removed. The molecular mass of the Japanese smelt AFP was calculated to be 16,756.8 by the TOF-mass analysis. The Open reading flame of the gene coding for the Japanese smelt AFP was 444 bp long and was 85.0% identical with the entire herring AFP gene. The cDNA and amino acid sequence of the Japanese smelt AFP were the same length as those of herring AFP.
社団法人日本農芸化学会の論文
2003-03-23