Construction of an Effective Host-Vector System for the Yeast Saccharomyces exiguus Yp74L-3
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概要
- 論文の詳細を見る
An effective host-vector system specific to the yeast Saccharomyces exiguus Yp74L-3 was constructed to promote the molecular genetic analyses for the yeast. To obtain a stable reversionless host strain, we constructed an S. exiguus strain carrying leu2 : : ScURA3 by disrupting the S. exiguus LEU2 gene with the S. cerevisiae URA3 gene. A vector plasmid unique to S. exiguus was subsequently developed by inserting both the LEU2 gene and an ARS cloned from S. exiguus into an Escherichia coli phagemid, pUC119. The vector constructed, pTH119 was able to transform the S. exiguus leu2 : : ScURA3 strain to Leu^+ efficiently. The stability of the vector in the S. exiguus host cells resembled that of a YRp-type vector in S. cerevisiae.
- 社団法人日本農芸化学会の論文
- 1999-05-23
著者
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HASEGAWA Shuichi
Department of Quantum Engineering and System Science, University of Tokyo
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Hasegawa S
Microbial Toxicology Laboratory Riken Institute:department Of Applied Biological Chemistry Tamagawa
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HISATOMI Taisuke
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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TAKAHASHI Kazutaka
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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OOMOTO Tetsuya
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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YAMASHITA Yasunori
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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TSUBOI Michio
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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Yamashita Yasushi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Tsuboi M
Dept. Biotechnol. Fac. Engineer. Fukuyama Univ.
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Tsuboi Michio
Department Of Biology Faculty Of Science Osaka City University
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Oomoto Tetsuya
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Hisatomi T
Dept. Biotechnol. Fac. Engineer. Fukuyama Univ.
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Hisatomi Taisuke
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Takahashi Kazutaka
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Yamashita Y
Dept. Biotechnol. Fac. Engineer. Fukuyama Univ.
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