DNA Sequence of the β-Isopropylmalate Dehydrogenase Gene and Phylogenetic Analysis of the Yeast Saccharomyces exiguus Yp74L-3
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概要
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The β-isopropylmalate dehydrogenase(LEU2) gene from a homothallic wild-type yeast, Saccharomyces exiguus Yp74L-3, was analyzed to estimate the phylogenetic position of this strain in yeasts. The β-isopropylmalate dehydrogenase gene of Yp74L-3 was first isolated as a clone complementing the lue2 mutation of Saccharomyces cerevisiae, and then confirmed to complement the haploid leu2 mutant derived from strain Yp74L-3 through genetic transformation. The nucleotide sequence of the cloned DNA revealed an open reading frame(ORF) encoding the β-isopropylmalate dehydrogenase composed of 365 amino acids. The β-isopropylmalate dehydrogenase coding sequence from the Yp74L-3 strain displayed 76.7% similarity to that of S.cerevisiae. Candidates for a UAS and a TATA-box in the 5′-upstream region and for a poly-A attachment site in the 3′-downstream region were found. A phylogenetic tree constructed from the nucleotide sequences of the β-isopropylmalate dehydrogenase coding regions revealed that Yp74L-3 is located between S.cerevisiae and the Kluyveromyces yeasts. The LEU2 gene cloned from Yp74L-3 will serve as an effective genetic marker for constructing the transformation system in S.exiguus Yp74L-3.
- 公益社団法人日本生物工学会の論文
- 1999-01-25
著者
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Tsuboi Michio
Department Of Biology Faculty Of Science Osaka City University
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Hisatomi Taisuke
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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HORIO KEN-ICHI
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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MIMOTO TATSUYA
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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Mimoto Tatsuya
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Horio Ken-ichi
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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