Identification of the Orotidine-5'-Phosphate Decarboxylase Gene and Development of a Transformation System in the Yeast Saccharomyces exiguus Yp74L-3
スポンサーリンク
概要
- 論文の詳細を見る
To investigate the uracil biosynthetic pathway of the yeast Saccharomyces exiguus Yp74L-3,uracil auxotrophic mutants were isolated. Using conventional genetic techniques, four mutant genes concerned in uracil biosynthesis were identified and denoted as ura1,ura2,ura3,and ura4. Mutations in the URA3 and URA4 genes were specifically selected with 5-fluoroorotic acid (5-FOA). Vector plasmids containing the URA3 gene and an autonomously replicating sequence (ARS) of S. cerevisiae produced sufficient amounts of Ura^+ transformants from the ura4 mutant of S. exiguus. This fact indicates that the S. exiguus URA4 gene encodes orotidine-5'-phosphate decarboxylase (OMP decarboxylase) and demonstrates that vector plasmids for S. cerevisiae are also usable in S. exiguus.
- 社団法人日本農芸化学会の論文
- 1998-11-23
著者
-
Tsuboi Michio
Department Of Biology Faculty Of Science Osaka City University
-
Tsuboi Michio
Department Of Biotechnology Faculty Of Engineering Fukuyama University
-
Hisatomi T
Dept. Biotechnol. Fac. Engineer. Fukuyama Univ.
-
Hisatomi Taisuke
Department Of Biotechnology Faculty Of Engineering Fukuyama University
-
KUROYANAGI Satoshi
Department of Biotechnology, Faculty of Engineering, Fukuyama University
-
Kuroyanagi S
Department Of Biotechnology Faculty Of Engineering Fukuyama University
関連論文
- Construction of an Effective Host-Vector System for the Yeast Saccharomyces exiguus Yp74L-3
- DEFECT OF MATING ABILITY BY ELONGATION OF THE DNA LENGTH OF CHROMOSOME III IN THE YEAST SACCHAROMYCES CEREVISIAE
- Physical Characterization of the Chromosomal DNA of the Yeast Saccharomyces exiguus
- DNA-Length Polymorphisms of Chromosome III in the Yeast Saccharomyces cerevisiae
- Sexual process in eight-spored ascus-forming yeast, Schizosaccharomyces japonicus II. Dependency of the sexual process on the carbon source
- Sexual process in eight-spored ascus-forming yeast, Schizosaccharomyces japonicus I. Culture medium for the synchronous sexual process
- Saccharomyces cerevisiaeにおけるホモタリズム遺伝子の胞子形成に及ぼす影響〔英文〕
- Effects of antimetabolites on flowering in Arabidopsis
- REPRODUCTIVE ISOLATION BETWEEN NEIGHBORING YEASTS IN THE GENUS SACCHAROMYCES
- DNA Sequence of the β-Isopropylmalate Dehydrogenase Gene and Phylogenetic Analysis of the Yeast Saccharomyces exiguus Yp74L-3
- Identification of the Orotidine-5'-Phosphate Decarboxylase Gene and Development of a Transformation System in the Yeast Saccharomyces exiguus Yp74L-3
- Isolation of Leu^- Mutants and Transformation with YEp13 Plasmid DNA in Saccharomyces exiguus(Microbiology & Fermentation Industry)
- Effect of cyclic 3',5'-adenosine monophosphate on the sporulation of Saccharomyces cerevisiae
- Genetic Analysis of the Non-Sporulating Phenotype of Brewer's Yeasts
- Effect of light on sexual flocculation in Schizosaccharomyces japonicus
- Sporulation of Spheroplasts in Saccharomyces cerevisiae