DNA-Length Polymorphisms of Chromosome III in the Yeast Saccharomyces cerevisiae
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概要
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The chromosome-sized DNAs of sporulation-deficient mutants, which had been isolated by mutagenizing spores of a homothallic diploid strain (MT98a-3D) of Saccharomyces cerevisiae, were analyzed by pulsed-field gel electrophoresis. While the size of chromosome III DNA of the parent strain was 450 kb, some mutants had one or more chromosome III DNAs of 350 kb, 450 kb, 530 kb and 630 kb. No size variation was observed for other chromosomes. Chromosome III DNAs of laboratory-stock strains, except MT98a-3D, were in the neighborhood of 350 kb. Size variation of chromosome III was observed at a high frequency in spore clones derived from MT98a-3D strain. The results suggest that DNA-length polymorphisms of chromosome III are generated by the loss or addition of a specific DNA unit of approximately 100 kb.
- 公益社団法人日本生物工学会の論文
- 1993-06-25
著者
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Yoda Akira
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Tsuboi Michio
Department Of Biology Faculty Of Science Osaka City University
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Hisatomi Taisuke
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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KONTANI TATSUYA
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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OHSHIRO SEIKI
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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YAGISHITA NOBUHARU
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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Ohshiro Seiki
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Kontani Tatsuya
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Yagishita Nobuharu
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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