A Novel Cryoprotective Protein (CRP) with High Activity from the Ice-nucleating Bacterium, Pantoea agglomerans IFO12686
スポンサーリンク
概要
- 論文の詳細を見る
The ice-nucleating bacterium, Pantoea agglomerans IFO12686,induces the cryoprotective protein (CRP) by cold acclimation at 12℃. The CRP was purified to apparent homogeneity by various chromatographies. We found that the purified CRP was a monomer of approximately 29,000 according to gel filtration chromatography and SDS-PAGE, and was a heat-stable protein. The CRP could protect freeze-labile enzymes, lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH) and isocitrate dehydrogenase (iCDH), against freezing-thawing denaturation. The activity of the CRP was about 3.5×10^4 times more effective than bovine serum albumin (BSA) and 2×10^6 times than COR26 from the ice-nucleating bacterium Pseudomonas fiuorescens KUIN-1. We confirmed that the CRP was a novel protein, as judged by the a different molecule mass from the already-known cryoprotectants, and has an high cryoprotective activity.
- 社団法人日本農芸化学会の論文
- 2001-04-23
著者
-
OBATA Hitoshi
Graduate School of Bioresources, Mie University
-
Obata H
Graduate School Of Bioresources Mie University
-
Obata H
Department Of Biotechnology Faculty Of Engineering Kansai University
-
Obata Hitoshi
Department Of Biotechnology Faculty Of Engineering Kansai University
-
Kawahara Hidehisa
Department of Biotechnology, Faculty of Engineering, Kansai University
-
Hidehisa Kawahara
Department Of Biotechnology Faculty Of Engineering Kansai University:high Technology Research Center
-
Kawahara Hidehisa
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
-
Obata Hitoshi
Graduate School Of Bioresources Mie Univ.
-
Obata Hitoshi
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol
-
YAMADE KAZUHIRO
Department of Biotechnology, Faculty of Engineering, Kansai University
-
KODA Noriko
Department of Biotechnology, Faculty of Engineering, Kansai University
-
ASAEDA Toshiaki
Department of Biotechnology, Faculty of Engineering, Kansai University
-
Koda Noriko
Department Of Biotechnology Faculty Of Engineering Kansai University
-
Asaeda Toshiaki
Department Of Biotechnology Faculty Of Engineering Kansai University
-
Yamade K
Department Of Biotechnology Faculty Of Engineering Kansai University
-
Yamade Kazuhiro
Department Of Biotechnology Faculty Of Engineering Kansai University:kansai University High Technolo
-
Kawaguchi H
Department Of Orthopaedic Surgery Graduate School Of Medicine The University Of Tokyo
-
Kawaguchi Hiroshi
Department Of Orthopaedic Surgery The University Of Tokyo
関連論文
- Distinct roles of Sox5, Sox6, and Sox9 in different stages of chondrogenic differentiation
- Identification of sequence polymorphisms in two sulfation-related genes, PAPSS2 and SLC26A2, and an association analysis with knee osteoarthritis
- Isolation of multidrug resistance associated protein like gene from lead hyperaccumulator common buckwheat and its lead detoxification ability
- Effect of chlorate on nitrification in longan plantation soil(Soil Biology)
- Comparison of Ni/Zn Accumulation Ability of Thlaspi japonicum from Three Different Areas in Hokkaido (Plant Nutrition)
- Decontamination of Chlorate in Longan Plantation Soils by Bio-Stimulation with Molasses Amendment (Soil Biology)
- Decontamination of Chlorate in Longan Plantation Soils by Bio-Stimulation with Sugar Amendment(Soil Biology)
- Cloning and Characterization of Phytochelatin Synthase from a Nickel Hyperaccumulator Thlaspi japonicum and Its Expression in Yeast
- Cloning and Characterization of Phytochelatin Synthase from a Nickel Hyperaccumulator Thlaspi japonicum and Its Expression in Yeast (Plant Nutrition)
- Distribution of Ni and Zn in the Leaves of Thlaspi japonicum Growing on Ultramafic Soil
- Distribution of Ni and Zn in the Leaves of Thlaspi japonicum Growing on Ultramafic Soil (Plant Nutrition)
- Differential Scanning Calorimetry of the Effects of Ca^ on the Thermal Unfolding of Pseudomonas cepacia Lipase(Biochemistry & Molecular Biology)
- Chemical Composition of Guttation Fluid and Leaves of Petasites japonicus v. giganteus and Polygonum cuspidatum Growing on Ultramafic Soil
- Structural Characterization of a New Acidic Ecopolysaccharide and Cyclic (1→2) β-Glucan Produced by Rhizobium huakuii Forming Nodules on Astragalus sinicus
- Cloning and Expression of the Thiobacillus Ferroxidans 3-Isopropylmalate Dehydrogenase Gene in Escherichia coli
- Identification of sequence polymorphisms of the COMP (cartilage oligomeric matrix protein) gene and association study in osteoarthrosis of the knee and hip joints
- Tailor-made tricalcium phosphate bone implant directly fabricated by a three-dimensional ink-jet printer
- Physiological role of bone morphogenetic proteins in osteogenesis
- Involvement of cyclic guanosine monophosphate-dependent protein kinase II in chondrocyte hypertrophy during endochondral ossification
- Properties of a Novel Extracellular Cell-free Ice Nuclei from Ice-nucleating Pseudomonas antarctica IN-74(Microbiology & Fermentation Technology)
- Purification and Properties of an Ice-Nucleating Protein from an Ice-Nucleating Bacterium, Pantoea ananatis KUIN-3
- Cloning of ZIP family metal transporter genes from the manganese hyperaccumulator plant Chengiopanax sciadophylloides, and its metal transport and resistance abilities in yeast(Plant Nutrition)
- Continual pH lowering and manganese dioxide solubilization in the rhizosphere of the Mn-hyperaccumulator plant Chengiopanax sciadophylloides(Plant Nutrition)
- BUILDING AN INTERNATIONAL NETWORK EXCHANGE PROGRAM OF EDUCATION AND RESEARCH FOR GRADUATE COURSE STUDENTS IN LIFE SCIENCE AND BIOTECHNOLOGY(LIFE SCIENCE AND BIOTECHNOLOGY,50th anniversary edition)
- Enhanced Growth and Nodule Occupancy of Red Kidney Bean and Soybean Inoculated with Soil Aggregate-Based Inoculant
- Improved Survival of Nutrient-Starved Cells of Rhizobium tropici CIAT899 in Acid Soil Associated with High AI^ and Mn^ Contents
- Free Radical Injury in Rice Leaf under Zn Deficiency
- Characterization of Mycorrhizas Formed by Glomus sp. on Roots of Hypernodulating Mutants of Lotus japonicus
- Isolation of Two Different Phenotypes of Mycorrhizal Mutants in the Model Legume Plant Lotus japonicus after EMS-Treatment
- Simple Method of Genomic DNA Extraction from Rhizobia in Dried Nodules of Phaseolus vulgaris for Strain Differentiation by PCR-Based DNA Fingerprinting
- 8-18 Effect of nutrient starvation on survival, stress resistance and competitive nodulation ability of Rhizobium tropici CIAT899.
- 8-9 The influence of mycorrhizal colonization on condensed tannin synthesis in root and shoot of Lotus japonicus.
- Basic Subsite Theory Assumptions May Not Be Applicable to Hydrolysis of Cellooligosaccharides by Almond β-Glucosidase
- Changes in the Level of Protein and Activity of Cu/Zn-Superoxide Dismutase in Zinc Deficient Rice Plant, Oryza sativa L.
- Role of Spermidine in the Ice-Nucleating Activity of the EIM from Erwinia uredovora KUIN-3
- Purification and Characterization of Extracellular Ice-nucleating Matter from Erwinia uredovora KUIN-3
- Effects of Polyamine on the Growth of Acidiphilium facilis 24R under Acidic Conditions(Microbiology & Fermentation Industry)
- Purification and Characterization of 3-Isopropylmalate Dehydrogenase from Thiobacillus thiooxidans
- Significance of the C-Terminal Domain of Erwinia uredovora Ice Nucleation-Active Protein(Ina U)
- Formation of Ice Nucleation-active Vesicles in Erwinia uredovora at Low Temperature and Transport of InaU Molecules into Shed Vesicles
- Cloning and Sequencing of an Ice Nucleation Active Gene of Erwinia uredovora
- Steady-State Inhibitory Kinetic Studies on the Ligand Binding Modes of Aspergillus niger Glucoamylase
- Guanidine Hydrochloride-Induced Denaturation of Pseudomonas cepacia Lipase
- Possibility for Discriminating Between Two Representative Non Two-state Thermal Unfolding Models of Proteins by DSC
- Thermal Unfolding of the Starch Binding Domain of Aspergillus niger Glucoamylase
- Purification and Characterization of a Calcium Carbonate-Crystal-Growth Inhibitor from Bacterium in Antarctic Lake Vanda(平成16年度 第50回低温生物工学会研究報告)
- Production of Two Types of Ice Crystal-Controlling Proteins in Antarctic Bacterium(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Type II Antifreeze Protein from a Mid-latitude Freshwater Fish, Japanese Smelt (Hypomesus nipponensis)(Biochemistry & Molecular Biology)
- Identification of a Novel Anti-ice-nucleating Polysaccharide from Bacillus thuringiensis YY529(Microbiology & Fermentation Technology)
- Identification of an Antifreeze Lipoprotein from Moraxella sp. of Antarctic Origin(Microbiology & Fermentation Technology)
- Neurologic Level Diagnosis of Cervical Stenotic Myelopathy
- Spinal stenosis in grade I degenerative lumbar spondylolisthesis : a comparative study of outcomes following laminoplasty and laminectomy with instrumented spinal fusion
- Vertebral hemangioma compressing the thoracic spinal cord : application of computer-aided navigation and intraoperative spinal sonography for surgery through anterior and posterior approaches
- Revision cervical spine surgery using transarticular or pedicle screws under a computer-assisted image-guidance system
- Can Laminoplasty Maintain the Cervical Alignment Even When the C2 Lamina Is Contained?
- Postoperative Expansion of Intramedullary High-Intensity Areas on T2-Weighted Magnetic Resonance Imaging After Cervical Laminoplasty
- Retroodontoid pseudotumor resected by a high cervical lateral approach in a rheumatoid arthritis patient : a case report
- Cervical myelopathy in an adult due to atlantoaxial subluxation associated with Down syndrome : a case study
- Image-guided surgery for cervical disorders in rheumatoid arthritis
- Association of Bone Metabolism Regulatory Factor Gene Polymorphisms With Susceptibility to Ossification of the Posterior Longitudinal Ligament of the Spine and Its Severity
- Computer-assisted anterior spinal surgery for a case of recurrent giant cell tumor
- Long-Term Results of Operative Treatment for Cervical Spondylotic Myelopathy in Patients With Athetoid Cerebral Palsy : An Over 10-Year Follow-Up Study
- Long-Term Results of Double-Door Laminoplasty for Cervical Stenotic Myelopathy
- Determination of Ultratrace Indium in Water Sample by Electrothermal Atomic Absorption Spectrometry after Preconcentration with Solvent Extraction and Back Extraction
- Response of the Ice-nucleating Bacterium Pantoea ananas KUIN-3 during Cold Acclimation(Microbiology & Fermentation Technology)
- A Novel Cryoprotective Protein (CRP) with High Activity from the Ice-nucleating Bacterium, Pantoea agglomerans IFO12686
- A Cold Acclimation Protein with Refolding Activity on Frozen Denatured Enzymes
- The Control of the Production and Secretion of Extracellular Ice-nucleating Material of Erwinia uredovora KUIN-3
- Purification and Characterization of a Novel Cold-regulated Protein from an Ice-nucleating Bacterium, Pseudomonas fluorescens KUIN-1
- Purification and Properties of an Aminopeptidase from a Protamine-degrading Marine Bacterium
- Microbial Transformation of Carbazole to Indole-3-acetic Acid by Flavobacterium sp. OCM-1
- Effects of Polyamines on the Ice-nucleating Activity of Erwinia uredovora KUIN-3
- Overproduction and Substrate Specificity of 3-Isopropylmalate Dehydrogenase from Thiobacillus ferrooxidans
- Purification and Characterization of a New Anti-Nucleating Protein Isolated from Acinetobacter calcoaceticus KINI-1
- Identification of a Compound in Chamaecyparis taiwanensis Inhibiting the Ice-nucleating Activity of Pseudomonas fluorescens KUIN-1
- Evaluation of Bacterial Injury by Image Analysis of Cell Motion
- Production of D-Glutamate from L-Glutamate with Glutamate Racemase and L-Glutamate Oxidase
- A Novel Process of Ethanol Production Accompanied by Extraction of Sugar in Cane Chips
- Continuous Alcohol Production from Starchy Materials with a Novel Immobilized Cell/Enzyme Bioreactor
- Regulatory Region of Expression of Thiobacillus ferrooxidans leuB Gene in Escherichia coli
- Isolation and Characterization of a Marine Ice-nucleating Bacterium, Pseudomonas sp. KUIN-5, Which Produces Cellulose and Secretes It in the Culture Broth
- Cryoprotection and Cryosterilization Effects of Type I Antifreeze Protein on E. coli cells
- The Metabolism of Cyclohexanol by Exophiala jeanselmei
- Aromatization of 4-Oxocyclohexanecarboxylic Acid to 4-Hydroxybenzoic Acid by Two Distinctive Desaturases from Corynebacterium cyclohexanicum : Identification of the Intermediate
- Antibacterial Effect of ω-Cyclopentane Fatty Acids Synthesized by Bacillus subtilis 626 from Cyclopentane Carboxylic Acid
- The Structures and Functions of Ice Crystal-Controlling Proteins from Bacteria(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Ice-nucleation Gene of Pseudomonas viridiflava KUIN-2 Coded on a Plasmid
- Expression of the Ice Nucleation Active Gene of a Novel Plasmid pNVR-1 from Pseudomonas viridiflava in Escherichia coli and Pseudomonas aeruginosa
- Properties of Cell-Free Ice Nuclei from Ice Nucleation-Active Pseudomonas fluorescens KUIN-1
- Effects of Zinc Deficiency on the Growth, Proteins, and Other Constituents of Yeast, Saccharomyces cerevisiae, Cells
- Isolation and Characterization of a Branhamella Strain That Degrades Bitumen in the Presence of n-Tetradecane
- Effects of Cd on the Fluxes of K^+ and H^+ in Excised Roots
- Effect of Cd on Plasma Membrane ATPase from Plant Roots Differing in Tolerance to Cd
- Stabilization of Astaxanthin by A Novel Biosurfactant Produced by Rhodotorula mucilaginosa KUGPP-1
- Studies on the Reduction of 2, 6-Dichlorophenolindophenol by 6-Substituted 3-Hydroxy-4-Pyrones, Using a Stopped-flow Method
- Measurement of Reaction Rate of 3-Hydroxy-2-pyrone with 2, 6-Dichlorophenolindophenol
- The determination of kojic acid using the stopped-flow method.
- Stopped-Flow Measurement of Reaction Rate of Dihydroxyfumaric Acid with 2, 6-Dichlorophenolindophenol
- Formation of Some Reductones on the Reaction of L-Ascorbic Acid with Sodium Nitrite
- Studies on the Reduction of 2, 6-Dichlorophenolindophenol by Reductic Acid, Using a Stopped-flow Method