Production of D-Glutamate from L-Glutamate with Glutamate Racemase and L-Glutamate Oxidase
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概要
- 論文の詳細を見る
We studied production of D-glutamate from L-glutamate using a bioreactor consisting of two columns of sequentially connected immobilized glutamate racemase (EC 5.1.1.3,from Bacillus subtilis IFO 3336) and L-glutamate oxidase (EC 1.4.3.11,from Streptomyces sp.X119-6) : L-glutamate was racemized by the glutamate racemase column, and then L-glutamate was oxidized by the L-glutamate oxidase column. Consequently only D-glutamate remained, and was easily separated from the α-ketoglutarate formed by anion-exchange chromatography. Both enzymes were highly stabilized by immobilization. The pH and temperature optima of immobilized glutamate racemase (pH 8,40℃) were similar to those of immobilized L-glutamate oxidase (pH 7,50℃). Accordingly, we connected the two columns tandemly to do both enzyme reactions under the same conditions. Actually 4.5 μmol of D-glutamate was produced and isolated from 10μmol of L-glutamate, about 90% of the theoretical yield.
- 社団法人日本農芸化学会の論文
- 1999-12-23
著者
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WATANABE Mayumi
Department of Immunology and Medical Zoology, Graduate School of Medicine, Niigata University
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SODA Kenji
Department of Biotechnology, Faculty of Engineering, Kansai University
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Soda Kenji
Department Of Biotechnology Faculty Of Engineering Kansai University
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OIKAWA Tadao
Department of Pediatrics, National Tochigi Hospital
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YAMADE KAZUHIRO
Department of Biotechnology, Faculty of Engineering, Kansai University
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Yamade Kazuhiro
Department Of Biotechnology Faculty Of Engineering Kansai University:kansai University High Technolo
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MAKIURA Hidemi
Department of Biotechnology, Faculty of Engineering, Kansai University
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KUSAKABE Hitoshi
Yamasa Corporation
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Makiura Hidemi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Kusakabe H
Yamasa Shoyu Co. Ltd. Chiba
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Oikawa Tadao
Department Of Biotechnology Faculty Of Engineering Kansai University:kansai University High Technolo
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Oikawa Tadao
Department Of Biotechnology Faculty Of Engineering Kansai University
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Watanabe Mayumi
Department Of Applied Chemistry Faculty Of Engineering Kobe University
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Watanabe Mayumi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Soda Kenji
Department Of Biotechnology Faculty Of Engineering Kansai University:kansai University High Technolo
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Soda Kenji
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama University
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