A New Enzymatic Method for L-Phenylalanine Synthesis Using Aminoacylase and Phenylalanine Dehydrogenase
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概要
- 論文の詳細を見る
An aminoacylase, inducibly formed in Bacillus thermoglucosidius grown with a synthetic compound, acetamidocinnamate, was used for enzymatic synthesis of L-phenylalanine from chloroacetamidocinnamate. The reaction system consisted of the hydrolysis of chloroacetamidocinnamate to phenylpyruvate by aminoacylase and the reductive amination of phenylpyruvate to L-phenylalanine by phenylalanine dehydrogenase. The coenzyme NADH consumed was regenerated by a coupled reaction with formate dehydrogenase. Under optimum conditions for L-phenylalanine production, more than 98% of the initially added chloroacetamidocinnamate was converted effectively to L-phenylalanine without appreciable decomposition or racemization.
- 社団法人日本農芸化学会の論文
- 1997-07-23
著者
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SODA Kenji
Department of Biotechnology, Faculty of Engineering, Kansai University
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Soda Kenji
Department Of Biotechnology Faculty Of Engineering Kansai University
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Cho H‐y
Korea Univ. Seoul Kor
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Cho Hong-yon
Department Of Food Sience And Biotechnology Graduate School Of Biotechnology Korea University
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Cho Hong-yon
Department Of Food Science And Biotechnology Graduate School Of Biotechnology Korea University
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Cho Hong-yon
Department Of Food And Biotechnology Korea University
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Soda Kenji
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama University
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