Effect of Culture Conditions on Production of 5-Aminolevulinic Acid by Recombinant Escherichia coli
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概要
- 論文の詳細を見る
Aminolevulinic acid (ALA) is formed by the enzyme ALA synthase (hemA gene). Then ALA is converted to Porphobilinogen (PBG) by the ALA dehydratase (hemB gene). For the overproduction of ALA, we used an Escherichia coli BL21(DE3) containing a hemA gene from Bradyrhzobium japonicum, which was created in our previous work. The effects of pH on the ALA synthase and ALA dehydratase were investigated. The ALA synthase and ALA dehydratase activities were dependent on the pH of the medium, with maximal activities occurring at pH 6.5 and 8.0 respectively. At pH 6.5, extracellular ALA reached 23 mM in a jar-fermenter. In addition, the effects of some nutritional factors, such as nitrogen source and the ratio of carbon to nitrogen (C/N) on the fermentative production of ALA were investigated. The highest ALA production was found with 8:1 of C/N ratio. Among various nitrogen sources, the tryptone might be a useful one for ALA production.
- 社団法人 日本農芸化学会の論文
- 2005-03-23
著者
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Jun Woo-jin
Department Of Food And Nutrition Chonnam National University
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Shin Dong-hoon
韓国
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Shin D‐h
Millimeter-wave Innovation Technology Research Center (mint) Dongguk University
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Shin Dong‐hoon
Department Of Food And Biotechnology Korea University
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Shin Dong-hoon
Department Of Preventive Medicine School Of Medicine & Institute For Medical Genetics Keimyung U
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Shin Dong-hoon
Millimeter-wave Innovation Technology Research Center (mint) Dongguk University
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Cho Hong
Dep. Of Food And Biotechnology Korea Univ.
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Cho Hong-yon
Dep. Of Food Technol. Graduate School Of Life Sciences And Biotechnology Korea Univ.
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Cho H‐y
Korea Univ. Seoul Kor
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Shin Dong-hoon
Department Of Food And Biotechnology Korea University
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Cho Hong
Graduate School Of Biotechnology Korea University
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Cho Hong-yon
Department Of Food Sience And Biotechnology Graduate School Of Biotechnology Korea University
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Hong B‐s
Department Of Food Science And Technology Graduate School Of Biotechnology Korea University
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HONG Bum-Shik
Department of Food Technology, Graduate School of Life Sciences and Biotechnology, Korea University
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LEE Dae-Hee
Department of Agricultural Biotechnology and The BioMAX Institute, Seoul National University
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Shin D‐h
Dep. Of Food And Biotechnology Korea Univ.
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Lee Dae-hee
Department Of Food Science And Technology Graduate School Of Biotechnology Korea University
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Cho Hong-yon
Department Of Food Science And Biotechnology Graduate School Of Biotechnology Korea University
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Hong Bumshik
Department Of Food And Biotechnology Korea University
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Cho Hong-yon
Department Of Food And Biotechnology Korea University
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SHIN Dong
Department of Food and Biotechnology, Korea University
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HONG Bum-Shik
Department of Food and Biotechnology, Korea University
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