Purification and Characterization of Antithrombotics from Syzygium aromaticum (L.) MERR. & PERRY

概要

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Two antithrombotic polysaccharides with relatively high molecular weight (HMW) and low molecular weight (LMW) were isolated from the flower buds of Syzygium aromaticum (L.) MERR. & PERRY (clove) by anionexchange chromatography, hydrophobic interaction column chromatography and size exclusion chromatography (LMW:EC-2B-IIIa-2, M.W.ca. 34000;HMW:EC-2C-Ia-2, M.W.ca. 103000). The LMW polysaccharide was mainly composed of Rha, Gal, GalA and Ara (molar %:24.1, 18.9, 18.0 and 17.9, respectively) with 10.8% of sulfate and 18.2% of protein. The HMW fraction consisted of Ara, Gal, Glc and Rha (molar %:26.0, 23.7, 17.5 and 12.4, respectively) with 15.4% of sulfate and 8.0% of protein. Both polysaccharides had the backbone of type I rhamnogalacturonan and the side chain of arabinan. Also, most of the sulfates were attached at the position 6 of 3-linked galactosyl residues. Compared to the antithrombotic activity of the HMW fraction (plasma clotting time of 145s in APTT assay), the LMW fraction displayed a slightly low activity (90s). However, animal studies indicated that crude LMW polysaccharide did not show acute toxicity, while the acute LD_<50> of the HMW fraction was approximately 2-fold lower than that of heparin.
公益社団法人日本薬学会の論文
2001-02-01