Isolation and Characterization of Alkyl Peroxy Radical Scavenging Compound from Leaves of Laurus nobilis

概要

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EtOH-soluble fraction from leaves of Laurus nobilis(bay leaves)possessed the highest alkyl peroxy radical(ROO・)scavenging activity among 120 kinds of herbs and edible plants, using the bioassay system which could determine the viability of Staphylococcus aureus 209p by ROO・ cytotoxicity. After EtOH-soluble fraction was partitioned with chloroform, ethylacetate, n-butanol and water, the ethylacetate-soluble fraction(L-EA)possessing the highest scavenging activity was further fractionated by Silica gel, Sephadex LH-20 and semi-preparative HPLC analysis on μ-Bondapak C_<18> reverse phase, and a major flavonol(L-EA-IIa-3-H2)in leaves of L. nobilis was isolated. According to the ultraviolet-visible absorption spectra, L-EA-IIa-3-H2 was thought to be 3, 5, 7, 3'-OH or 3(5), 7, 3', 4'-OH flavonol. After acid hydrolysis of the fraction, L-EA-IIa-3-H2 was found to consist of quercetin and glucose, and was confirmed by one- or two-dimensional(1D or 2D)-NMR to be isoquercitrin. In addition, the ROO・ scavenging activity of L-EA-IIa-3-H2 was supported by ESR and its activity was found to be comparable to that of other well-known antioxidants such as epigallocatechin and resveratrol, and higher than that of butylated hydroxyanisole(BHA), butylated hydroxytoluene(BHT)and ascorbic acid.
公益社団法人日本薬学会の論文
2002-01-01