Formation of Ice Nucleation-active Vesicles in Erwinia uredovora at Low Temperature and Transport of InaU Molecules into Shed Vesicles

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概要

• 論文の詳細を見る

• An ice nucleation-active strain of Erwinia uredovora shed vesicles when cultured at low temperature (10℃). We isolated an ice nucleation-active vesicle fraction from the culture medium by ultrafiltration, ultracentrifugation, and gel filtration. Western blot analysis showed that this cell-free vesicle fraction contained an ice nucleation-active protein (InaU). The process of the InaU transport to a shed vesicle was examined by immunohistochemical analysis using electron microscopy. The examination showed the following successive processes : InaU molecules first assemble around the inner membrane, then the assembly enters a vesicle just forming on the surface of the outer membrane, and finally the vesicle, 100-400 nm in diameter, leaves the surface to be shed with InaU molecules occluded .

• 社団法人日本農芸化学会の論文
• 1995-10-23

著者

• Abe K

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Obata H

  Department Of Biotechnology Faculty Of Engineering Kansai University

• Obata Hitoshi

  Department Of Biotechnology Faculty Of Engineering Kansai University

• Abe K

  Univ. Tokyo Tokyo Jpn

• Abe Keiko

  Agricultural And Life Sciences Dep. Of Applied Biological Chemistry

• Arai S

  Tokyo Univ. Agriculture Tokyo Jpn

• Arai S

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Arai S

  Department Of Applied Biological Chemistry The University Of Tokyo

• Arai Soichi

  Department Of Applied Biological Chemistry The University Of Tokyo

• Arai Soichi

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Arai Soichi

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Science The

• Abe K

  Univ Tokyo Tokyo Jpn

• MICHIGAMI Yasuyuki

  Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Science, T

• Abe Keiko

  Research & Development Department, Nakano Vinegar Co., Ltd.

• Iwabuchi Kyoko

  Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences,

• Arai Soichi

  Faculty of Engineering, Kansai University

• Arai Soichi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Arai Soichi

  Department Of Nutrition Tokyo University Of Agriculture

• Abe K

  Faculty Of Pharmacy Musashino University

• Arai S

  Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University

• Arai Soichi

  Faculty Of Education Tokyo Gakugei University

• Obata Hitoshi

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol

• Iwabuchi Kyoko

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Michigami Y

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Science The

• Michigami Yasuyuki

  Department Of Agricultural Chemistry The University Of Tokyo

• Arai Soichi

  Faculty Of Applied Biological Sciences Tokyo University Of Agriculture

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