Guanidine Hydrochloride-Induced Denaturation of Pseudomonas cepacia Lipase.
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概要
- 論文の詳細を見る
The guanidine hydrochloride-induced denaturation of Pseudomonas cepacia lipase (PCL) was studied at pH 7 by monitoring the changes in the fluorescence and circular dichroism of the enzyme. The denaturation was irreversible as a whole, and the addition of Ca2+ ions decreased the velocity of the denaturation. The denaturation process was well explained consistently by a two-step mechanism, as follows: where N is the native state of PCL, D1 an intermediate denatured-state which can be refolded into the native state, and DF the final denatured-state that can not be renatured. Ethanol (10%) increased the denaturation velocity by decreasing the refolding step, DI+Ca2+→N•Ca2+, which would be caused by the stabilization of DI by ethanol.
- 社団法人 日本生化学会の論文
著者
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Senoo Keishi
Faculty Of Agriculture The University Of Tokyo
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Inouye Kuniyo
Graduate School Of Agriculture Kyoto University
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Okuda Kozue
Faculty Of Bioresources Mie University
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Tanaka Akiyoshi
Faculty Of Bioresources Mie University
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Obata Hitoshi
Faculty Of Agriculture Mie University
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Obata Hitoshi
Faculty of Bioreources, Mie University
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