Fluorometric Study of the Acid-induced Denaturation of Staphylococcal Nuclease and Its Mutant Forms
スポンサーリンク
概要
- 論文の詳細を見る
The acid-induced denaturation of wild-type Staphylococcal nuclease (WT) and its eight mutant forms, L25A, V66L, G79S, A90S, G88V, H124L, V66L/G88V, and V66L/G79S/G88V, was investigated using Trp140 fluorescence as a probe at 30 °C. The values of pH1⁄2, at which the denaturation is half completed, and n, the apparent number of protons which trigger the denaturation and are taken up by the proteins upon denaturation at pH1⁄2, were evaluated from the pH dependence of the fluorescence intensity. The values of pH1⁄2 and n for WT were 3.8 and 1.8 respectively. The amino acid replacements changed the pH1⁄2 values to a range between 3.0 (H124L) and 4.4 (G79S) and also changed the n values to a range between 1.0 (A90S) and 3.0 (G88V). There was a negative correlation between the values of pH1⁄2 and n. It was suggested that the amino acid replacements may change the energy levels of the native state and/or the denatured state mainly in the neutral (stable) pH region, not in the acidic (unstable) region, resulting in the correlative changes in pH1⁄2 and n.
- 社団法人 日本農芸化学会の論文
- 2004-06-23
著者
-
田中 晶善
三重大学生物資源学部
-
田中 晶善
三重大学 教育
-
Tanaka Akiyoshi
Faculty Of Bioresources Mie University
-
田中 晶善
三重大学生物資源学研究科
関連論文
- 9-28 マンガン集積性植物コシアブラのカルス再分化に伴うマンガン集積と集積関連遺伝子の探索(9.植物の無機栄養)
- 熱測定法による食塩の防腐効果の定量的解析
- 8-10 ミヤコグサ(Lotus japnicus)樹枝状体超着生変異体(Arb^)からの樹枝状体の単離(8.共生)
- マメ科モデル植物・ミヤコグサLotus japonicusの共生変異体の単離と解析
- 8-10 ミヤコグサ(Lotus japonicus)ハイパーノジュレーション変異体に形成されるアーバスキュラー菌根樹枝状体の特徴(8.共生)
- 8-10 アーバスキュラー菌根の共生しないミヤコグサ(Lotus japonicus)変異体の特徴(8.共生)
- 8-3 アーバスキュラー菌根の共生しないミヤコグサ(Lotus japonicus)変異体の探索(8.共生)
- Decontamination of Chlorate in Longan Plantation Soils by Bio-Stimulation with Sugar Amendment(Soil Biology)
- Cloning and Characterization of Phytochelatin Synthase from a Nickel Hyperaccumulator Thlaspi japonicum and Its Expression in Yeast
- Cloning and Characterization of Phytochelatin Synthase from a Nickel Hyperaccumulator Thlaspi japonicum and Its Expression in Yeast (Plant Nutrition)