Substrate-dependent Activation of Thermolysin by Salt
スポンサーリンク
概要
- 論文の詳細を見る
Salt-activation of thermolysin was examined using a positively charged fluorescent substrate, (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 [MOCAc-PLGL(Dpa)AR]. Thermolysin activity increased in a biphasic exponential fashion and was 40 times higher in the presence of 4 M NaCl than in its absence. The degree of activation at x M NaCl was expressed as 4.7x when [NaCl]o<0.5 M and 2.3x when [NaCl]o>0.5 M respectively.
- 社団法人 日本農芸化学会の論文
- 2004-08-23
著者
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Inouye K
Kyoto Univ. Kyoto Jpn
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Inoue K
Kansai Medical Univ. Osaka
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INOUYE Kuniyo
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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ONEDA Hiroshi
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Oneda H
Kyoto Univ. Kyoto Jpn
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Oneda Hiroshi
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Muta Y
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Muta Yuko
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Inouye K
Kyoto Univ. Kyoto
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Inouye Kuniyo
Division If Applied Life Sciences Graduate School Of Agriculture Kyoto University
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