Refolding and Recovery of Recombinant Human Matrix Metalloproteinase 7 (Matrilysin) from Inclusion Bodies Expressed by Escherichia coli
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概要
- 論文の詳細を見る
- Japanese Biochemical Societyの論文
- 1999-11-01
著者
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INOUYE Kuniyo
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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ONEDA Hiroshi
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Oneda H
Kyoto Univ. Kyoto Jpn
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Oneda Hiroshi
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Inouye Kuniyo
Division If Applied Life Sciences Graduate School Of Agriculture Kyoto University
関連論文
- Two Crystal Structures of Lysyl-tRNA Synthetase from Bacillus stearothermophilus in Complex with Lysyladenylate-Like Compounds : Insights into the Irreversible Formation of the Enzyme-Bound Adenylate of L-Lysine Hydroxamate
- Analysis of Autodegradation Sites of Thermolysin and Enhancement of Its Thermostability by Modifying Leu^ at an Autodegradation Site
- Effects of Nitration and Amination of Tyrosyl Residues in Thermolysin on Its Hydrolytic Activity and Its Remarkable Activation by Salts
- Insights into the Catalytic Roles of the Polypeptide Regions in the Active Site of Thermolysin and Generation of the Thermolysin Variants with High Activity and Stability
- Effects of Site-directed Mutagenesis of the Surface Residues Gln128 and Gln225 of Thermolysin on its Catalytic Activity
- Improvement in Performance of Affinity Gels Containing Gly-D-Phe as a Ligand to Thermolysin Due to Increasing the Spacer Chain Length
- Engineering of the pH-Dependence of Thermolysin Activity as Examined by Site-Directed Mutagenesis of Asn112 Located at the Active Site of Thermolysin
- Effects of High Concentration of Salts on the Esterase Activity and Structure of a Kiwifruit Peptidase, Actinidain
- Comparison of the Wild-Type α-Amylase and Its Variant Enzymes in Bacillus amyloliquefaciens in Activity and Thermal Stability, and Insights into Engineering the Thermal Stability of Bacillus α-Amylase
- Comparison of Starch Hydrolysis Activity and Thermal Stability of Two Bacillus licheniformis α-Amylases and Insights into Engineering α-Amylase Variants Active under Acidic Conditions
- Lysyl-tRNA Synthetase of Bacillus stearothermophilus Molecular Cloning and Expression of the Gene
- Development of a Synchronous Enzyme-Reaction System for a Highly Sensitive Enzyme Immunoassay
- State of Tryptophyl Residues and Stability of Recombinant Human Matrix metalloproteinase 7 (Matrilysin) as Examined by Fluorescence^1
- Antioxidative Effects of Glycosyl-ascorbic Acids Synthesized by Maltogenic Amylase to Reduce Lipid Oxidation and Volatiles Production in Cooked Chicken Meat
- Insight into the Mechanism of the Stabilization of Moloney Murine Leukaemia Virus Reverse Transcriptase by Eliminating RNase H Activity
- Characterization of Moloney Murine Leukaemia Virus/Avian Myeloblastosis Virus Chimeric Reverse Transcriptases
- Effects of Neutral Salts and Alcohols on the Activity of Streptomyces caespitosus Neutral Protease
- Purification to Homogeneity of a Neutral Metalloproteinase from Streptomyces caespitosus
- Comparison of the Thermal Stabilities of Reverse Transcriptases from Avian Myeloblastosis Virus and Moloney Murine Leukaemia Virus
- Effects of Site-Directed Mutagenesis of the Loop Residue of the N-Terminal Domain Gly117 of Thermolysin on Its Catalytic Activity
- Inhibitory Effects of Green Tea Catechins on the Activity of Human Matrix Metalloproteinase 7 (Matrilysin)
- Effects of Organic Solvents on the Reverse Transcription Reaction Catalyzed by Reverse Transcriptases from Avian Myeloblastosis Virus and Moloney Murine Leukemia Virus
- Inhibitory Effects of 1, 4-Naphthoquinone Derivatives on Rat Cytochrome P4501A1-Dependent Monooxygenase Activity in Recombinant Yeast Microsomes
- Total Synthesis of a Natural Antioxidant and Structure-Activity Relationships of Related Compounds
- Expression in Escherichia coli, Refolding, and Purification of the Recombinant Mature Form of Human Matrix Metalloproteinase 7 (MMP-7)
- Detection of Antigen-antibody Reaction Using a Fluorescent Probe and Its Application to Homogeneous Competitive-type Immunoassay for Insulin
- Purification and Characterization of Purple Acid Phosphatase PAP1 from Dry Powder of Sweet Potato(Biochemistry & Molecular Biology)
- Inhibitory Effects of Alcohols on the Activity of Human Matrix Metalloproteinase 7 (Matrilysin)
- Substrate-dependent Activation of Thermolysin by Salt
- Refolding and Recovery of Recombinant Human Matrix Metalloproteinase 7 (Matrilysin) from Inclusion Bodies Expressed by Escherichia coli
- Effect of Nitration on the Activity of Bovine Erythrocyte Cu, Zn-Superoxide Dismutase (BESOD) and a Kinetic Analysis of Its Dimerization-Dissociation Reaction as Examined by Subunit Exchange between the Native and Nitrated BESODs
- Inhibitory Effects of Alcohols on Thermolysin Activity as Examined Using a Fluorescent Substrate
- Emulsifying Properties of a Peptide from Peptic Hydrolysates of Soy Glycinin
- Inhibitory Effect of 0.19 α-Amylase Inhibitor from Wheat Kernel on the Activity of Porcine Pancreas α-Amylase and Its Thermal Stability
- Need for Aromatic Residue at Position 115 for Proteolytic Activity Found by Site-directed Mutagenesis of Tryptophan 115 in Thermolysin
- Analysis of the Mechanism of Inhibition of Human Matrix Metalloproteinase 7 (MMP-7) Activity by Green Tea Catechins
- Comparison of the Thermal Stabilities of the αβ Heterodimer and the α Subunit of Avian Myeloblastosis Virus Reverse Transcriptase
- Effects of Organic Solvents on the Reverse Transcription Reaction Catalyzed by Reverse Transcriptases from Avian Myeloblastosis Virus and Moloney Murine Leukemia Virus
- Insight into the Mechanism of the Stabilization of Moloney Murine Leukaemia Virus Reverse Transcriptase by Eliminating RNase H Activity
- Improvement in Performance of Affinity Gels Containing Gly-D-Phe as a Ligand to Thermolysin Due to Increasing the Spacer Chain Length
- Purification to Homogeneity of a Neutral Metalloproteinase from Streptomyces caespitosus
- Expression in Escherichia coli, Refolding, and Purification of the Recombinant Mature Form of Human Matrix Metalloproteinase 7 (MMP-7)
- Effects of Site-Directed Mutagenesis of the Loop Residue of the N-Terminal Domain Gly117 of Thermolysin on Its Catalytic Activity
- Chemiluminescent Enzyme Immunoassay for Measuring Leptin
- Activity of Glucose-6-phosphate 1-Dehydrogenase in Hair Follicles with Male-pattern Alopecia
- Catalytic Properties of an Organic Solvent-Resistant Tyrosinase from Streptomyces sp. REN-21 and Its High-Level Production in E. coli
- Effects of Cobalt-Substitution of the Active Zinc Ion in Thermolysin on Its Activity and Active-Site Microenvironment
- Kinetic Analysis of the Activation-and-Inhibition Dual Effects of Cobalt Ion on Thermolysin Activity
- Molecular Mechanism of the Inhibitory Effect of Cobalt Ion on Thermolysin Activity and the Suppressive Effect of Calcium Ion on the Cobalt Ion-dependent Inactivation of Thermolysin
- Effects of Dimethyl Sulfoxide, Temperature, and Sodium Chloride on the Activity of Human Matrix Metalloproteinase 7 (Matrilysin)^1
- Interactions of Human Matrix Metalloproteinase 7 (Matrilysin) with the Inhibitors Thiorphan and R-941381
- Effect of Salts on the Solubility of Thermolysin: A Remarkable Increase in the Solubility as Well as the Activity by the Addition of Salts without Aggregation or Dispersion of Thermolysin^1
- Lysyl-tRNA Synthetase from Bacillus stearothermophilus.Stopped-Flow Kinetic Analysis of Enzyme・Lysyladenylate Formation
- Analysis of the Mechanism of Inhibition of Human Matrix Metalloproteinase 7 (MMP-7) Activity by Green Tea Catechins
- Comparison of the Thermal Stabilities of the αβ Heterodimer and the α Subunit of Avian Myeloblastosis Virus Reverse Transcriptase
- Effects of Polyethylene Glycol on Bovine Intestine Alkaline Phosphatase Activity and Stability
- Effects of Polyethylene Glycol on Bovine Intestine Alkaline Phosphatase Activity and Stability
- Stabilization of Bovine Intestine Alkaline Phosphatase by Sugars
- Tyr219 of human matrix metalloproteinase 7 is not critical for catalytic activity, but is involved in the broad pH-dependence of the activity
- Effects of Conversion of the Zinc-Binding Motif Sequence of Thermolysin, HEXXH, to That of Dipeptidyl Peptidase III, HEXXXH, on the Activity and Stability of Thermolysin
- Effects of Mutations of Thermolysin, Asn116 to Asp and Asp150 to Glu, on Salt-Induced Activation and Stabilization
- Refolding and Recovery of Recombinant Human Matrix Metalloproteinase 7 (Matrilysin) from Inclusion Bodies Expressed by Escherichia coli.
- A possibility of a protein-bound water molecule as the ionizable group responsible for pKe at the alkaline side in human matrix metalloproteinase 7 activity
- Effects of site-directed mutagenesis of Asn116 in the β-hairpin of the N-terminal domain of thermolysin on its activity and stability
- Interaction of 8-anilinonaphthalene 1-sulphonate (ANS) and human matrix metalloproteinase 7 (MMP-7) as examined by MMP-7 activity and ANS fluorescence