Detection of Antigen-antibody Reaction Using a Fluorescent Probe and Its Application to Homogeneous Competitive-type Immunoassay for Insulin
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概要
- 論文の詳細を見る
The interaction of bovine insulin with anti-human insulin antibody (mAb) was examined using a fluorescent probe. The fluorescence intensity of fluoresceinthiocarbamyl (FTC)-insulin was increased by adding mAb, and the increase was saturated at 53% at a molar ratio of FTC-insulin to mAb of 2.0. Based on the change in fluorescence intensity, a standard curve of the homogeneous competitive-type immunoassay was constructed, and the detection range of insulin was found to be 50–400 nM.
- 社団法人 日本農芸化学会の論文
- 2004-10-23
著者
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INOUYE Kuniyo
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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ONEDA Hiroshi
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Oneda Hiroshi
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Oneda Hiroshi
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Inouye Kuniyo
Division Of Applied Life Sciences Graduate School Of Agricultur Kyoto University
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Inouye Kuniyo
Division If Applied Life Sciences Graduate School Of Agriculture Kyoto University
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