Insight into the Mechanism of the Stabilization of Moloney Murine Leukaemia Virus Reverse Transcriptase by Eliminating RNase H Activity
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概要
- 論文の詳細を見る
We explored the mechanism of the stabilization of Moloney murine leukaemia virus reverse transcriptase (MMLV RT) by eliminating RNase H activity. Without the template-primer (T/P) poly(rA)-p(dT)15, the temperature reducing initial reverse-transcription activity by 50% over a 10-min incubation of the RNase H activity-deficient variant D524A was higher by 3.7 °C than that of the wild-type enzyme (WT). In the reverse transcription reaction, the Km values for T/P of WT and D524A were almost the same. These results suggest that elimination of RNase H activity enhanced the intrinsic thermal stability of MMLV RT rather than its affinity toward T/P.
- 社団法人 日本農芸化学会の論文
- 2010-02-23
著者
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Inouye Kuniyo
Div. Of Food Sci. And Biotechnology Graduate School Of Agriculture Kyoto Univ. Sakyo-ku Kyoto 606-85
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YASUKAWA Kiyoshi
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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INOUYE Kuniyo
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Yasukawa Kiyoshi
Div. Of Food Sci. And Biotechnology Graduate School Of Agriculture Kyoto Univ.
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Yasukawa Kiyoshi
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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MIZUNO Masaki
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Mizuno Masaki
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Inouye Kuniyo
Division If Applied Life Sciences Graduate School Of Agriculture Kyoto University
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