Comparison of the Thermal Stabilities of the αβ Heterodimer and the α Subunit of Avian Myeloblastosis Virus Reverse Transcriptase
スポンサーリンク
概要
- 論文の詳細を見る
Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63-kDa α subunit and a 95-kDa β subunit. In this study, we explored the role of the interaction between the α and β subunits on AMV RT stability. The recombinant AMV RT α subunit was expressed in insect cells and purified. It exhibited lower thermal stability than the native AMV RT αβ heterodimer. Unlike the αβ heterodimer, the α subunit was not stabilized by template-primer. These results suggest that interaction between the α and β subunits is important for AMV RT stability.
- 社団法人 日本農芸化学会の論文
著者
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YASUKAWA Kiyoshi
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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INOUYE Kuniyo
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Yasukawa Kiyoshi
Div. Of Food Sci. And Biotechnology Graduate School Of Agriculture Kyoto Univ.
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Yasukawa Kiyoshi
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Inouye Kuniyo
Division Of Applied Life Sciences Graduate School Of Agricultur Kyoto University
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NEMOTO Daisuke
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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KONISHI Atsushi
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Nemoto Daisuke
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Inouye Kuniyo
Division If Applied Life Sciences Graduate School Of Agriculture Kyoto University
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