Purification to Homogeneity of a Neutral Metalloproteinase from Streptomyces caespitosus
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概要
- 論文の詳細を見る
We established an improved purification procedure for Streptomyces caespitosus neutral protease (ScNP) from culture supernatants of S. caespitosus. The procedure comprises sequential ammonium sulfate fractionation and column chromatography procedures with anion exchange chromatography, followed by hydrophobic-interaction chromatography and gel filtration. Purified ScNP revealed a single band with a molecular mass of 14 kDa by SDS–PAGE under reduced conditions and did not contain any detectable pigment, which has not been completely removed by other methods. We also purified another protease with a molecular mass of 40 kDa from the culture supernatants. The pure preparation of ScNP obtained by this procedure is suitable for spectrophotometric measurement of its catalytic activity.
- 社団法人 日本農芸化学会の論文
著者
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Inouye Kuniyo
Kyoto Univ. Kyoto City Jpn
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Inouye Kuniyo
Div. Of Food Sci. And Biotechnology Graduate School Of Agriculture Kyoto Univ. Sakyo-ku Kyoto 606-85
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SHIMADA Takashi
Division of Clinical Genetics, Nippon Medical School Hospital
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YASUKAWA Kiyoshi
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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INOUYE Kuniyo
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Yasukawa Kiyoshi
Div. Of Food Sci. And Biotechnology Graduate School Of Agriculture Kyoto Univ.
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