D-Alanine-D-Alanine Ligase Gene (ddl) of Erwinia chrysanthemi Strain EC16 l. Isolation and Gene Dosage Effect on Pectate Lyase Synthesis
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概要
- 論文の詳細を見る
New regulatory gene for pectate lyase (Pel) production of Erwinia chrysanthemi strain EC16 was searched by observing the gene dosage effect of each cosmid library in Pel production. From this survey, a cosmid clone, p5A, had reduced in Pel production under both inducing and non-inducing conditions and caused less tissue maceration of potato tubers. The 2.64-kb HindIII fragment from p5A was found to be responsible for this phenotype and to contain one major ORF consisting of 1107 nucleotides, which had homology with ddlA (49.6%) and ddlB (49.6%) of Escherichia coli and with ddlA (44.6%) of Salmonella typhimurium. These genes had been shown to encode D-alanine-D-alanine ligase (DdI), an enzyme which is involved in the synthesis of peptidoglycan. This ORF encodes 368 amino acid residues and its molecular weight is estimated to be 43 kDa. This ORF of EC16 could complement ddl deficient mutant of Escherichia coli. (Received May 15, 2002 ; Accepted June 20, 2002)
- 日本植物病理学会の論文
- 2002-12-25
著者
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Matsumoto Hiroyuki
Tokyo Institute Of Technology
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Tsuyumu S
Laboratory Of Plant Pathology Faculty Of Agriculture Shizuoka University
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Tsuyumu Shinji
Laboratory Of Plant Pathology Faculty Of Agriculture Shizuoka University
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UMEHARA Masahiro
Faculty of Agriculture, Shizuoka University
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JITAREERAT Pongphen
Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University
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UMEHARA Masahiro
Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University
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JITAREERAT Pongphen
The United Graduate School of Agricultural Science,Gifu University
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MATSUMOTO Hiroyuki
The United Graduate School of Agricultural Science,Gifu University
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Matsumoto H
Tokyo Institute Of Technology Department Of Mechanical Engineering And Science
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Umehara Masahiro
Laboratory Of Plant Pathology Faculty Of Agriculture Shizuoka University
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Jitareerat Pongphen
The United Graduate School Of Agricultural Science Gifu University:laboratory Of Plant Pathology Fac
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