Complex Endosymbiotic Microbiota of the Citrus Psyllid Diaphorina citri (Homoptera : Psylloidea)(Phylogeney)
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概要
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We investigated the endosymbiotic microbiota of the citrus psyllid Diaphorina citri that vectors Liberobacter sp., the causative agent of citrus greening disease. Eubacterial 16S rDNA fragments were amplified by PCR and cloned from the whole DNA of D. citri. RFLP analysis showed that the PCR products contained five major sequence types. Nucleotide sequences of the five types were determined and subjected to molecular phylogenetic analysis. The first sequence was a member of the γ-Proteobacteria, closely related to mycetocyte symbionts of other psyllids. The second sequence belonged to the β-Proteobacteria, related to Oxalobacter and Herbaspirillum. The third sequence, belonging to the γ-Proteobacteria, showed a significant similarity to the sequences of Arsenophonus spp., known as endosymbionts of a triatomine bug and a parasitoid wasp. The fourth sequence was almost identical to the sequences of Liberobacter spp. in the a-Proteobacteria. The fifth sequence showed a high similarity to the sequences of Wolbachia spp. in the α-Proteobacteria. In situ hybridization experiments using specific oligonucleotide probes confirmed that the first and second sequences originated from the mycetocyte and syncytium symbionts, respectively. The presence/ absence of the five endosymbiotic bacteria in adult individuals of D. citri from an Indonesian natural population was examined by diagnostic PCR analysis. The mycetocyte symbiont and the syncytium symbiont exhibited 100% infection, whereas the Arsenophonus, Liberobacter and Wolbachia showed partial infections at a rate of 83.3%, 45.2% and 76.2%, respectively.
- 社団法人日本動物学会の論文
- 2000-09-20
著者
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Somowiyarjo Susamto
Department Of Entomology And Plant Pathology Faculty Of Agriculture Gadjah Mada University
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Subandiyah S
Gadjah Mada Univ. Yogyakarta Idn
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Subandiyah Siti
Department Of Entomology And Plant Pathology Faculty Of Agriculture Gadjah Mada University
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Nikoh Naruo
National Institute of Bioscience and Human-Technoloav, Aaency of Industrial Science and Technology
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Tsuyumu Shinji
Faculty of Agriculture, Shizuoka University
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Fukatsu Takema
National Institute of Bioscience and Human-Technoloav, Aaencv of Industrial Science and Technology
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Nikoh N
Univ. The Air Chiba Jpn
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Tsuyumu S
Laboratory Of Plant Pathology Faculty Of Agriculture Shizuoka University
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Tsuyumu Shinji
Faculty Of Agriculture Shizuoka University
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Fukatsu T
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Fukatsu Takema
National Institute Of Advanced Industrial Science And Technology
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Fukatsu Takema
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Scinece A
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Fukatsu T
National Institute Of Bioscience And Human-technology Agency Of Industrial Science And Technology
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Fukatsu T
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Scinece A
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Fukatsu Takema
National Inst. Advanced Industrial Sci. And Technol.(aist) Tsukuba Jpn
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Fukatsu Takema
National Inst. Advanced Industrial Sci. And Technol.(aist) Ibaraki Jpn
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Fukatsu Takema
National Inst. Advanced Industrial Sci. And Technol. (aist) Tsukuba Jpn
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