High Prevalence of Wolbachia in the Azuki Bean Beetle Callosobruchus chinensis (Coleoptera, Bruchidae)(Ecology)
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概要
- 論文の詳細を見る
Members of the family Bruchidae (Insecta, Coleoptera) were examined for Wolbachia infection. Out of seven species investigated, which represented three subfamilies and five genera, amplified bands were detected only from Callosobruchus chinensis by diagnostic PCR with Wolbachia-specific primers. Bacterial 16S rDNA was amplified by PCR, cloned and sequenced from the total DNA of C. chinensis. Molecular phylogenetic analysis demonstrated that the sequence belongs to a monophyletic group of the genus Wolbachia in the α-Proteobacteria. The Wolbachia was detected from all tissues and body parts such as ovary, gut, fat body, muscle, wing, leg, head and antenna. In six geographic populations from central Japan, all the individuals examined, more than 400 in total, possessed the Wolbachia, indicating high prevalence of Wolbachia among natural populations of C. chinensis.
- 社団法人日本動物学会の論文
- 1999-12-15
著者
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KONDO Natsuko
Departments of Biology, School of Medicine, Nara Medical University
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Kondo N
National Inst. Advanced Industrial Sci. And Technol. Tsukuba Jpn
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Kondo Natsuko
Department Of Biology School Of Medicine Nara Medical University
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Shimada Masakazu
Department of Systems Sciences, the University of Tokyo
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Fukatsu Takema
National Institute of Bioscience and Human-Technoloav, Aaencv of Industrial Science and Technology
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Fukatsu Takema
National Institute Of Bioscience And Human-technology Agency Of Industrial Science And Technology
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Fukatsu Takema
National Inst. Advanced Industrial Sci. And Technol.(aist) Tsukuba Jpn
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Shimada M
Hokkaido Univ. Sapporo Jpn
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Shimada Masakazu
Department Of Systems Sciences (biology) The University Of Tokyo
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Fukatsu Takema
National Inst. Advanced Industrial Sci. And Technol.(aist) Ibaraki Jpn
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Shimada Masakazu
Department Of Biology College Of Arts And Sciences The University Of Tokyo
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Fukatsu Takema
National Inst. Advanced Industrial Sci. And Technol. (aist) Tsukuba Jpn
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