Nitric Oxide Radical-induced Radioadaptation and Radiosensitization Are G2/M Phase-dependent

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概要

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• The aim of this study was to examine biological effects of nitric oxide (NO) on radiosensitivity and chromosome aberrations in different phases of the cell cycle in human cancer cells with a wild-type p53 (wtp53) genotype. H1299/wtp53 cells were pre-treated with isosorbide dinitrate (ISDN) at different concentrations or pre-irradiated with a low dose of X-rays, and then exposed to a high dose of X-rays. Cell synchronization was achieved with serum starvation. Cellular radiosensitivity, cell cycle distributions, and chromosome aberrations were assayed with colony-forming assays, flow cytometry and chromosome banding techniques, respectively. After treatment with ISDN at a low concentration or after an exposure to 0.02 Gy of X-rays, radioresistance and a reduction in the number of chromosome aberrations were observed mainly 17.5 h after plating mitotic cells. This radioadaptation effect was observed during a clearly shortened G2/M phase and a slightly prolonged S phase. In contrast, in the presence of a high concentration of ISDN, radiosensitization and the enhancement of chromosome aberrations were detected principally 17.5 h after plating mitotic cells, and this radiosensitization was observed during a significantly prolonged G2/M phase and a slightly shortened S phase. A range of concentrations of NO induced opposing effects on radiosensitivity and chromosome aberrations in human non-small cell lung cancer cells bearing wtp53 gene status, and these different effects produced by NO depended on the cell cycle phase.

著者

• Kondo Natsuko

  Department Of Biology School Of Medicine Nara Medical University

• Ohnishi Takeo

  Department Of Biology Nara Medical University

• Su Xiaoming

  Department Of Biology School Of Medicine Nara Medical University

• Nakagawa Yosuke

  Department Of Oral And Maxillofacial Surgery School Of Medicine Nara Medical University

• Iwasaki Toshiyasu

  Radiation Safety Research Center Nuclear Technology Research Laboratory Central Research Institute O

• Takahashi A

  Department Of Biology Nara Medical University

• GUO Guozhen

  Department of Radiation Medicine, Fourth Military Medical University

• Guo Guozhen

  Department Of Radiation Medicine Fourth Military Medical University

• TAKAHASHI Akihisa

  Advanced Scientific Research Leaders Development Unit, Gunma University

• KONDO Natsuko

  Particle Radiation Oncology Research Center, Research Reactor Institute, Kyoto University

• Takahashi Akihisa

  Advanced Scientific Research Leaders Development Unit Gunma University

• Kondo Natsuko

  Particle Radiation Oncology Research Center Research Reactor Institute Kyoto University

• IWASAKI Toshiyasu

  Radiation Safety Research Center, Nuclear Technology Research Laboratory, Central Research Institute of the Electric Power Industry of Japan

• NAKAGAWA Yosuke

  Department of Oral and Maxillofacial Surgery, School of Medicine, Nara Medical University

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