Live-Cell-Driven Insertion of a Nanoneedle
スポンサーリンク
概要
- 論文の詳細を見る
We demonstrated the insertion of an intracellular nanoneedle into a single living cell which was caused by a cell-driven force. The cell-driven assembly of bio-hybrid micro and nanocomponents offers a potential route in nano-bioengineering research on new fabrication tools of bio-micro electro-mechanical systems (bio-MEMS) without direct mechanical manipulation. We describe the cell-driven self-insertion of a single conductive tungsten nanoneedle of 190 nm diameter. A single nanoneedle was fabricated on the bottom of each 15 μm wide glass microwell. A myoblast C2C12 cell migrated into a microwell; within 3 hours, the insertion of a nanoneedle into the cell was observed using a three-dimensional laser scanning confocal microscope. Observation of fluorescence labeled viable cells indicated that no dead cells were present in the microwells during the nanoneedle insertion process. The intracellular nanoneedles were broken after a maximum of 2 days.
- 2009-10-25
著者
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HOSHINO Takayuki
Department of Mechanical Systems Engineering, Tokyo University of Agriculture and Technology
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Konno Tomohiro
Department Of Materials Engineering And Bioengineering School Of Engineering University Of Tokyo
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Ishihara Kazuhiko
Department Of Biochemistry Kitasato University Graduate School Of Medical Sciences
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Morishima Keisuke
Department of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
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Hoshino Takayuki
Department of Mechanical Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
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Ishihara Kazuhiko
Department of Materials Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Hoshino Takayuki
Department of Clinical Research, Oriental Medicine Research Center, Kitasato University, Japan
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HOSHINO Takayuki
Department of Agricultural Chemistry, The University of Tokyo
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