Characterization of Rat Hair Follicle Stem Cells Selected by Vario Magnetic Activated Cell Sorting System
スポンサーリンク
概要
- 論文の詳細を見る
Hair follicle stem cells (HfSCs) play crucial roles in hair follicle morphogenesis and hair cycling. These stem cells are self-renewable and have the multi-lineage potential to generate epidermis, sebaceous glands, and hair follicle. The separation and identification of hair follicle stem cells are important for further research in stem cell biology. In this study, we report on the successful enrichment of rat hair follicle stem cells through vario magnetic activated cell sorting (Vario MACS) and the biological characteristics of the stem cells. We chose the HfSCs positive surface markers CD34, α6-integrin and the negative marker CD71 to design four isolation strategies: positive selection with single marker of CD34, positive selection with single marker of α6-integrin, CD71 depletion followed by CD34 positive selection, and CD71 depletion followed by α6-integrin positive selection. The results of flow cytometry analysis showed that all four strategies had ideal effects. Specifically, we conducted a series of researches on HfSCs characterized by their high level of CD34, termed CD34bri cells, and low to undetectable expression of CD34, termed CD34dim cells. CD34bri cells had greater proliferative potential and higher colony-forming ability than CD34dim cells. Furthermore, CD34bri cells had some typical characteristics as progenitor cells, such as large nucleus, obvious nucleolus, large nuclear:cytoplasmic ratio and few cytoplasmic organelles. Our findings clearly demonstrated that HfSCs with high purity and viability could be successfully enriched with Vario MACS.
著者
-
Yang Li
Department Of Biotechnology Graduate School Of Agicultural And Life Sciences The University Of Tokyo
-
Chen Wei
Department Of Chemical Engineering Nagoya University
-
Lian Xiaohua
Department of Cell Biology, Third Military Medical University
-
Yang Tian
Department of Cell Biology, Third Military Medical University
-
Huang Enyi
Department of Bioengineering, Chongqing University
-
Chen Wei
Department of Cell Biology, Third Military Medical University
-
Yang Li
Department of Bioengineering, Chongqing University
関連論文
- Endothelin-1 regulates rat bone sialoprotein gene transcription
- Effects of progesterone receptor modulator CDB2914 on apoptosis of cultured uterine leiomyoma cells(Oncology 12)
- 7-24.Direct Effects of GnRH Antagonist (Cetrorelix) on Proliferative Activity, Apoptosis and EGF Expression in Cultured Human Uterine Leiomyoma Cells(Session 9 Others 1)
- Magnetization plateaus in one dimensional S=1/2 Heisenberg model with dimerization and quadrumerization
- Ground State Properties of One Dimensional S=1/2 Heisenberg Model with Dimerization and Quadrumerization
- SAGE library screening reveals ILT7 as a specific plasmacytoid dendritic cell marker that regulates type I IFN production
- Characterization of pEC01, a Novel Plasmid Required for Phosphate Taxis in Enterobacter cloacae IFO 3320
- Critical Properties of Spin-1 Antiferromagnetic Heisenberg Chains with Bond Alternation and Uniaxial Single-Ion-Type Anisotropy
- Bond operator mean field approach to the magnetization plateaux in quantum antiferromagnets - Application to the S=1/2 coupled dimerized zigzag Heisenberg chains
- Magnetization plateaus in antiferromagnetic-(ferromagnetic), polymerized S=1/2 XXZ chains
- Ground State Phase Diagram of the One Dimensional S=1/2 XXZ Model with Dimerization and Quadrumerization
- Hand-assisted versus pure laparoscopic radical cystectomy : A clinical outcome comparison
- Hand-assisted laparoscopic radical cystectomy and extracorporeal urinary diversion : Experience with 31 cases
- Isolation and Characterization of the Enterobacter cloacae cheR Mutant Defective in Phosphate Taxis
- Lipopolysaccharide administration enhances hypoxic-ischemic brain damage in newborn rats
- Lipopolysacharide administration induces brain damage after intermittent hypoxia in the newborn rat(Session19 Peninatology4)
- Enzymatic Conversion of Dehydrodivanillin to Vanillin by an Anaerobic Recombinant FE7
- Anaerobic Degradation of Dehydrodiisoeugenol by Rumen Bacteria
- Detection of Potato Virus Y P1 Protein in Infected Cells and Analysis of Its Cleavage Site
- Mutational Analysis of the Potato Virus Y 5' Untranslated Region for Alteration in Translational Enhancement in Tobacco Protoplasts
- A Parallel Method for the Prefix Convex Hulls Problem
- Simultaneous Voltammetric Determination of Ascorbic Acid, Dopamine and Uric Acid Using Polybromothymol Blue Film-Modified Glassy Carbon Electrode
- A Polymer Film Modified Sensor for Voltammetric Determination of Uric Acid in the Presence of Ascorbic Acid and Its Application in Urine
- LATERAL AND AXIAL MIXING OF THE DISPERSED PARTICLES IN CFB
- Polynomially Fast Parallel Algorithms for Some P-Complete Problems (Special Section on Discrete Mathematics and Its Applications)
- Low Temperature Synthesis of Ultrafine Sn Te Powder in Ethanol Solvent
- Improving Ability of Married Women to Prevent Reproductive Tract Infections in Rural Western China
- Knockdown of focal adhesion kinase reverses colon carcinoma multicellular resistance
- Molecular phylogeny of species in the genera Amylostereum and Echinodontium
- Expression of inducible nitric oxide synthase and cyclooxygenase-2 mRNA in brain damage induced by lipopolysaccharide and intermittent hypoxia-ischemia in neonatal rats
- 11-23.Decreased variability is associated with brain damage induced by lipopolysaccharide and intermittent hypoxiaischemia in 7-day-old rats (Session 13 Perinatology 2)
- GABA_C-Receptor Stimulation Activates cAMP-Dependent Protein Kinase via A-Kinase Anchoring Protein 220
- Transjugular Intrahepatic Portosystemic Shunts : Midterm Results Results in 28 Patients
- Therapeutic potential and related signal pathway of adipose-derived stem cell transplantation for rat liver injury
- Effects of Xylooligosaccharides in Type 2 Diabetes Mellitus
- Alternative lengthening of telomeres in hTERT-inhibited laryngeal cancer cells
- Content of Selected Flavonoids in 100 Edible Vegetables and Fruits
- Band-Bending at the Graphene–SiC Interfaces: Effect of the Substrate
- Hsa-mir-27a genetic variant contributes to gastric cancer susceptibility through affecting miR-27a and target gene expression
- High Barrier Height Nickel/GaAs Schottky Diodes Fabricated by Addition of Pr_2O_3 into a Ga Melt for Liquid Phase Epitaxy
- Designing Efficient Parallel Algorithms with Multi-Level Divide-and-Conquer (Special Section on Discrete Mathematics and Its Applications)
- Butyric acid stimulates bone sialoprotein gene transcription
- 20-P2-CJK07 DISSOLUTION COMPARISONS FOR NIMODIPINE TABLETS FROM DIFFERENT FACTORIES
- Performance of tiloronoxim and tilorone determination in human blood by HPLC-MS/MS : Method validation, uncertainty assessment and its application to a pharmacokinetic study
- Simultaneous quantification of tiloronoxim and tilorone in human urine by liquid chromatography-tandem mass spectrometry
- Renormalization Group Potential for Quasi-One-Dimensional Correlated Systems(Yukawa International Seminar 2004 (YKIS2004) Physics of Strongly Correlated Electron Systems)
- Finding the Envelope of Segments in Parallel
- P-14 8-Chloro-Adenosine-Liposomes and Its Properties
- Electrochemical Oxidation of Luteolin at a Glassy Carbon Electrode and Its Application in Pharmaceutical Analysis
- Derivation of Hepatocytes From Injected Bone Marrow cells in Mice After Partia Hepatectomy and D-Galactosamine Induced Hepatic Injury(THE SIXTH JAPAN-CHINA JOINT SEMINAR ON HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Characterization of Rat Hair Follicle Stem Cells Selected by Vario Magnetic Activated Cell Sorting System
- Establishment of an Experimental Mouse Model of Trauma-Hemorrhagic Shock
- Fibroblast growth factor 2 and forskolin induce mineralization-associated genes in two kinds of osteoblast-like cells
- Changes in heart rate patterns by lipopolysaccharide and intermittent hypoxia-ischemia in 7-day-old rats
- A Leakage-Aware CS/CB Scheme for Heterogeneous CoMP Networks with Layered Limited Feedback
- GABAC-Receptor Stimulation Activates cAMP-Dependent Protein Kinase via A-Kinase Anchoring Protein 220
- Expression Pattern of Cyclooxygenase-2 in Normal Rat Epidermis and Pilosebaceous Unit during Hair Cycle
- High Barrier Height Nickel/GaAs Schottky Diodes Fabricated by Addition of Pr2O3 into a Ga Melt for Liquid Phase Epitaxy
- A fuzzy evaluation of schedule robustness under processing time variations in batch plants.
- Study on the T-helper cell 1/2 cytokine profile in blister fluid of patients with herpes zoster and its clinical significance
- Effect of the regulation of retinoid X receptor-α gene expression on rat hepatic fibrosis
- Effect of prednisone on transforming growth factor-β1, connective tissue growth factor, nuclear factor-kBp65 and tumor necrosis factor-α expression in a murine model of hepatic sinusoidal obstruction syndrome induced by Gynura segetum