Quantification and improvement of error rate during ligase detection reaction(METHODS)
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概要
- 論文の詳細を見る
We estimated the actual error rate during ligase detection reaction (LDR), and confirmed that DNA sequences around 3' ends are greatly influenced on the specificity of LDR. Its specificity is increased about 1000 times by introducing a mismatch base near the 3' ends.
著者
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Ginya Harumi
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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Yohda Masafumi
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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Matsushita Ryouhei
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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Ginya Harumi
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology:precisio
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Matsushita Ryouhei
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
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Yohda Masafumi
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
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Yohda Masafumi
Department Of Biotechnology And Life Science Graduate School Of Engineering Tokyo University Of Agri
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