Development of an Automation System for Single Nucleotide Polymorphisms Genotyping Using Bio-Strand, a New Three-Dimensional Microarray (Medical Biotechnology)
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概要
- 論文の詳細を見る
Previously, we developed a novel three-dimensional microarray system called Bio-Strand, which may be used in various applications including single nucleotide polymorphisms genotyping. In Bio-Strand, samples for detection are immobilized on a one-dimensional thread, which is wound around a cylinder-shaped core to form a three-dimensional thread-and-core structure. The thread-and-core structure is then inserted into a plastic pipette tip, where hybridization and detection are performed. In this study, we have developed an automation system, NIAGALA Bio-Station SDx, which enables automated hybridization and detection during the genotyping procedure using Bio-Strand. Using this system, we have performed the single nucleotide polymorphism (SNP) genotyping of CYP2C, one of the important human cytochrome P450 genes and the results were completely consistent with the genotyping results determined by the TaqMan method.
- 社団法人日本生物工学会の論文
- 2005-02-25
著者
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Asahina Junko
Precision System Science Co., Ltd.
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Takahashi Masaaki
Precision System Science Co., Ltd.
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Yohda Masafumi
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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Asahina Junko
Precision System Science Co. Ltd.
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Asahina Junko
東京農工大学 生命工学専攻
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TOJO YURIKO
Precision System Science Co., Ltd.
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MIYASHITA YUKIKO
Precision System Science Co., Ltd.
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MATSUMOTO NAOKO
Sekino Clinical Pharmacology Clinic
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HASEGAWA SETSUO
Sekino Clinical Pharmacology Clinic
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TAJIMA HIDEJI
Precision System Science Co., Ltd.
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Tajima Hideji
Precision Systems Science Co. Ltd.
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Yohda M
Tokyo Univ. Agriculture And Technol. Tokyo
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Yohda Masafumi
Department Of Biotechnology And Life Science Graduate School Of Engineering Tokyo University Of Agri
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Yohda M
Inst. Physical And Chemical Res. (riken) Saitama Jpn
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Miyashita Yukiko
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
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Tojo Yuriko
Precision System Science Co. Ltd.:department Of Biotechnology And Life Science Tokyo University Of A
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Matsumoto Naoko
Sekino Clinical Pharmacology Clinic:department Of Biotechnology And Life Science Tokyo University Of
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Yoshida Mamiko
Precision Systems Science Co. Ltd.
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Takahashi Masaaki
Precision System Science Co. Ltd.
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