Integration of the Mutated Biotin Biosynthetic Genes to the Chromosome of a d-Biotin-Producing Strain of Serratia marcescens
スポンサーリンク
概要
- 論文の詳細を見る
To construct a genetically stable strain of Serratia marcescent for d-biotin production, we integrated the mutated biotin-biosynthetic (bio) genes into the chromosome of a d-biotin-producing strain of S. marcescens Sr41. Temperature-sensitive suicide vectors consisting of the Tn5 element were used as the integration tool. Extra bio genes carried between the Tn5 termini were integrated into the chromosome of strain ETA23,a d-biotin-producing mutant. Resultant integrants produced 120 mg of d-biotin per l of a medium containing sucrose and urea whereas ETA23 produced d-biotin at 55 mg/l in the medium. The integrated bio genes were stably mainained on the chromosome of ETA23 for 100 generations.
- 社団法人日本生物工学会の論文
- 1994-06-25
著者
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Komatsubara Saburo
(present Address)research Laboratory Of Applied Biochemistry At Toda Tanabe Seiyaku Co. Ltd.
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Tosa Tetsuya
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.
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Tosa Tetsuya
Research Laboratory Of Applied Biochemistry Tanbe Seiyaku Co. Ltd.
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Tosa Tetsuya
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Imai Yuji
Research Laboratory Of Applied Biochemistry Tanbe Seiyaku Co. Ltd.
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SAKURAI NAOKI
(Present address)Research Laboratory of Applied Biochemistry at Toda, Tanabe Seiyaku Co. Ltd.,
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Sakurai Naoki
(present Address)research Laboratory Of Applied Biochemistry At Toda Tanabe Seiyaku Co. Ltd.
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