Industrial production of L-alanine from ammonium fumarate using immobilized microbial cells of two kinds.
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概要
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Rate equations were derived for a two-step enzyme reaction which produced L-alanine from ammonium fumarate via L-aspartic acid by aspartase of immobilized Escherichia coli cells and L-aspartate β-decarboxylase of immobilized Pseudomonas dacunhae cells. To establish an efficient system for industrial production of L-alanine, we investigated the design of adequate bioreactors on the basis of the simulation of this sequential reaction by solving the simultaneous equations derived. As a result, L-alanine was found to be produced efficiently by use of two sequential column reactors: a conventional column reactor containing immobilized E. coli cells and a closed column reactor containing immobilized P. dacunhae cells.
- 公益社団法人 化学工学会の論文
著者
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Chibata Ichiro
Research Laboratories Tanabe Seiyaku Co. Ltd.
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Tosa Tetsuya
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Takamatsu Satoru
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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