Hyperproduction of Aspartase by a Catabolite Repression-Resistant Mutant of Escherichia coli B Harboring Multicopy aspA and par Recombinant Plasmids

スポンサーリンク

概要

• 論文の詳細を見る

• Recombinant plasmids bearing the Escherichia coli K-12 aspartase gene(aspA) and the plasmid partition locus (par) were introduced into a catabolite repression-resistant strain of E. coli B, AT202,constructed by mutational and transdectional methods. Plasmid pNK101(pBR322-aspA-par) was stably maintained in cells of AT202 even after 30 cell generations, while pYT471(pBR322-aspA), which bore bo par locus, was lost at high frequencies from the host cells. Strain AT202 harboring pNK101 produced 3-fold and 80-fold more aspartase than the wild-type E. coli B harboring pNK101 and the wild-type E. coli B strain, respectively. The maximum amount of aspA peoduct (aspartase) was 40-45% of the total cellular protein.

• 公益社団法人日本生物工学会の論文
• 1989-02-25

著者

• Komatsubara Saburo

  Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.

• Nishimura Noriyuki

  Graduate School Of Integrated Science Yokohama City University

• Nishimura Noriyuki

  Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.

• Nishimura N

  Graduate School Of Art And Science Yokohama City University

• TANIGUCHI TOMOYASU

  Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.,

• Taniguchi Tomoyasu

  Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.

関連論文

• APPLICATION OF TRANSDUCTION TO "METABOLICALLY CONTROLLED FERMENTATION"-CONSTRUCTION OF AMINO ACIDPRODUCING STRAINS
• Isolation and Characterization of Novel Mutants Affecting the Abscisic Acid Sensitivity of Arabidopsis Germination and Seedling Growth
• A Novel Ethanol-Hypersensitive Mutant of Arabidopsis
• A Novel Arabidopsis Gene Required for Ethanol Tolerance is Conserved Among Plants and Archaea
• Purification and Some Properties of Alanine Aminotransferase from Candida maltosa
• ABA Hypersensitive Germination2-1 Causes the Activation of Both Abscisic Acid and Salicylic Acid Responses in Arabidopsis
• Hyperproduction of Aspartase by a Catabolite Repression-Resistant Mutant of Escherichia coli B Harboring Multicopy aspA and par Recombinant Plasmids
• Mechanism of Coenzyme A Biosynthesis by Sarcina lutea
• Synthetic studies on phosphorylating reagent. V. A convenient synthesis of 2',3'-cyclic Coenzyme A.

スポンサーリンク