Hyperproduction of Aspartase by a Catabolite Repression-Resistant Mutant of Escherichia coli B Harboring Multicopy aspA and par Recombinant Plasmids
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概要
- 論文の詳細を見る
Recombinant plasmids bearing the Escherichia coli K-12 aspartase gene(aspA) and the plasmid partition locus (par) were introduced into a catabolite repression-resistant strain of E. coli B, AT202,constructed by mutational and transdectional methods. Plasmid pNK101(pBR322-aspA-par) was stably maintained in cells of AT202 even after 30 cell generations, while pYT471(pBR322-aspA), which bore bo par locus, was lost at high frequencies from the host cells. Strain AT202 harboring pNK101 produced 3-fold and 80-fold more aspartase than the wild-type E. coli B harboring pNK101 and the wild-type E. coli B strain, respectively. The maximum amount of aspA peoduct (aspartase) was 40-45% of the total cellular protein.
- 公益社団法人日本生物工学会の論文
- 1989-02-25
著者
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Komatsubara Saburo
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Nishimura Noriyuki
Graduate School Of Integrated Science Yokohama City University
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Nishimura Noriyuki
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Nishimura N
Graduate School Of Art And Science Yokohama City University
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TANIGUCHI TOMOYASU
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.,
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Taniguchi Tomoyasu
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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