Synthetic studies on phosphorylating reagent. V. A convenient synthesis of 2',3'-cyclic Coenzyme A.
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概要
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A convenient method has been developed for the preparation of 2′,3′-cyclic Coenzyme A (<B>5</B>). It was readily prepared in a good yield from the reaction of adenosine-5′ 2-dimethylamino-4-nitrophenyl phosphate (<B>3</B>) with D-pantethine-4′,4″-diphosphate (<B>4</B>) in the presence of acetic acid in pyridine. Compound <B>3</B> and the diphosphate <B>4</B> were prepared in a good yield from the condensation of adenosine (<B>1</B>) with 2-dimethylamino-4-nitrophenyl phosphate (<B>2</B>) in the presence of dicyclohexylcarbodiimide (DCC) and the phosphorylation of D-pantethine with 2, respectively.<BR>Phosphate <B>5</B> was hydrolyzed with 0.1 M-hydrochloric acid and subsequently reduced with 2-mercaptoethanol to confirm its structure of <B>5</B>. One of the products, Coenzyme A (reduced form) (<B>6</B>) showed 102% activity with phosphotransacetylase.
- 公益社団法人 日本化学会の論文
著者
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Nishimura Noriyuki
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Kakimoto Toshio
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Nishimura Noriyuki
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.
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Taguchi Yoshihiko
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.
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Mushika Yoshitaka
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.
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- Synthetic studies on phosphorylating reagent. V. A convenient synthesis of 2',3'-cyclic Coenzyme A.