Universal Processing Technique for Protein Crystals Using Pulsed UV Laser
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概要
- 論文の詳細を見る
We demonstrated the laser processing of three types of soft protein crystals using a 193 nm deep-UV pulsed laser. In particular, high-solvent-content crystals, phosphoenolpyruvate carboxylase (PEPC) crystals, were successfully processed without causing significant damage. An unchanged resolution limit between pre- and post-processings in X-ray diffraction measurements showed that laser irradiation has little influence on crystallinity. These results reveal that the processing using a deep-UV laser is effective for various protein crystals and will be very useful in accelerating protein structural analysis.
- Japan Society of Applied Physicsの論文
- 2004-07-01
著者
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Takano Kazufumi
SOSHO Project (Crystal Design Project), Osaka University
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Matsumura Hiroyoshi
SOSHO Project (Crystal Design Project), Osaka University
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Inoue Tsuyoshi
SOSHO Project (Crystal Design Project), Osaka University
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Doi Masaaki
Core Technology Center Nikon Corporation
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Kitano Hiroshi
Department Of Electrical Engineering Graduate School Of Engineering Osaka University:core Technology
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Mori Yusuke
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Adachi Hiroaki
Department Of Biological Science Faculty Of Science Hiroshima University
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Sasaki Takatomo
Department Of Electrical Electronic And Information Engineering Osaka University
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Murakami Atsutoshi
Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Kitano Hiroshi
Department of Chemistry, Faculty of Science, Shizuoka University
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