Novel and Simple Screening Methods for Protein Crystallization by Vapor Diffusion Rate Control
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概要
- 論文の詳細を見る
We investigated the effect of the vapor diffusion rate on the refinement of protein crystals. The reservoir volume, drop-to-reservoir distance, and air space were altered to change the vapor diffusion rate using a very dense insoluble liquid and specific crystallization plates. The experimental results reveal that the drop-to-reservoir distance and air space have a more significant effect on crystal growth than the reservoir volume. We propose new simple and useful methods for screening protein crystal growth conditions based on vapor diffusion rate control.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2004-09-15
著者
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Takano Kazufumi
SOSHO Project (Crystal Design Project), Osaka University
-
Matsumura Hiroyoshi
SOSHO Project (Crystal Design Project), Osaka University
-
Inoue Tsuyoshi
SOSHO Project (Crystal Design Project), Osaka University
-
Niino Ai
SOSHO Inc.
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Mori Yusuke
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Adachi Hiroaki
Department Of Biological Science Faculty Of Science Hiroshima University
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Sasaki Takatomo
Department Of Electrical Electronic And Information Engineering Osaka University
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