Improving the Quality of Protein Crystals Using Stirring Crystallization
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概要
- 論文の詳細を見る
Recent reports state that a high magnetic field improves the crystal quality of bovine adenosine deaminase (ADA) with an inhibitor [Kinoshita et al.: Acta Cryst. D59 (2003) 1333]. In this paper, we examine the effect of stirring solution on ADA crystallization using a vapor-diffusion technique with rotary and figure-eight motion shakers. The probability of obtaining high-quality crystals is increased with stirring in a figure-eight pattern. Furthermore, rotary stirring greatly increased the probability of obtaining high-quality crystals, however, nucleation time was also increased. The crystal structure with the inhibitor was determined at a high resolution using a crystal obtained from a stirred solution. These results indicate that stirring with simple equipment is as useful as the high magnetic field technique for protein crystallization.
- Japan Society of Applied Physicsの論文
- 2004-04-15
著者
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Takano Kazufumi
SOSHO Project (Crystal Design Project), Osaka University
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Matsumura Hiroyoshi
SOSHO Project (Crystal Design Project), Osaka University
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Inoue Tsuyoshi
SOSHO Project (Crystal Design Project), Osaka University
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Niino Ai
SOSHO Inc.
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WARIZAYA Masaichi
Exploratory Research Laboratories, Fujisawa Pharmaceutical Co., Ltd.
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Kinoshita Takayoshi
Exploratory Research Laboratoeis Fujisawa Pharmaceutical Co. Ltd.
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Mori Yusuke
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Adachi Hiroaki
Department Of Biological Science Faculty Of Science Hiroshima University
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Sasaki Takatomo
Department Of Electrical Electronic And Information Engineering Osaka University
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Kinoshita Takayoshi
Exploratory Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., 5-2-3 Tokodai, Tsukuba, Ibaraki 300-2698, Japan
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Takano Kazufumi
SOSHO Project (Crystal Design Project), Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Warizaya Masaichi
Exploratory Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., 5-2-3 Tokodai, Tsukuba, Ibaraki 300-2698, Japan
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