Application of UV-Laser Ablation to Detaching Protein Crystal from Growth Vessel
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概要
- 論文の詳細を見る
We propose a new procedure for detaching protein crystals from a growth vessel, which we call harvesting adhered crystals by laser irradiation (HACLI). A single laser shot at a wavelength of 193 nm successfully detaches hen egg-white lysozyme crystals from a fused-silica glass plate or capillary tube. X-ray diffraction measurements demonstrate that laser irradiation has little influence on the crystallinity. The HACLI technique will be a powerful tool for handling fragile protein crystals, especially those grown in a glass capillary tube.
- Japan Society of Applied Physicsの論文
- 2004-10-01
著者
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Takano Kazufumi
SOSHO Project (Crystal Design Project), Osaka University
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Matsumura Hiroyoshi
SOSHO Project (Crystal Design Project), Osaka University
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Inoue Tsuyoshi
SOSHO Project (Crystal Design Project), Osaka University
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Doi Masaaki
Core Technology Center Nikon Corporation
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Kitano Hiroshi
Department Of Electrical Engineering Graduate School Of Engineering Osaka University:core Technology
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Mori Yusuke
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Adachi Hiroaki
Department Of Biological Science Faculty Of Science Hiroshima University
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Sato Atsushi
Department Of Applied Chemistry Chubu University
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Sasaki Takatomo
Department Of Electrical Electronic And Information Engineering Osaka University
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Kitano Hiroshi
Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Murakami Atsutoshi
Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Sato Atsushi
Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Sasaki Takatomo
Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Doi Masaaki
Core Technology Center, Nikon Corporation, 1-6-3 Nishi-Ohi, Shinagawa-ku, Tokyo 140-8601, Japan
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Kitano Hiroshi
Department of Chemistry, Faculty of Science, Shizuoka University
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