N-(Benyloxycarbony)-L-Aspartyl-L-Phenylalanine Methyl Ester Production by Immobilized Nocardia opaca : Thermolysin under Hydrogen High Pressure in a Water-Organic Solvent System

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概要

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• N-(Benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester (Z-APM) has been produced from phenylpyruvate methyl ester (PPyOMe) and N-(benzyloxycarbonyl)-aspartic acid (Z-Asp) under hydrogen high pressure in a water-ethyl acetate system. Z-APM was produced in a two step reaction usign immobilized Nocardia opaca and thermolysin. Phenylalanine methyl ester (PheOMe) was produced from PPyOMe, NH_4Cl and H_2 by immobilized N. opaca. The PheOMe production rate increased linearly with increasing hydrogen pressure. The PheOMe production rate was 0.41μmol/min/g dry cells under 100 atm of hydrogen. Z-APM was produced from PheOMe and Z-Asp by immobilized thermolysin. THe highest activity(22.2μmol/min/g thermolysin)was obtained by coupling N. opaca immobilized in calcium alginate and termolysin immobilized in photo-crosslinkable resin prepolymer ENTP-4000. When repeated use was carried out without reactivation of the immobilized N. opaca, Z-APM production was not observed after triple use. Therefore, immobilized N. opaca was separated from immobilized thermolysin and reactivated periodically in nutrient broth for 20 h. As a result, the immobilized system retained 72% of the initial activity after being used 5 times for over 100 h.

• 社団法人日本生物工学会の論文
• 1989-06-25

著者

• Tsuji Masao

  Kuraray Co.

• MATSUNAGA Tadashi

  Department of Pediatric Surgery, Chiba University School of Medicine

• Nakamura Noriyuki

  Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

• Matsunaga Tadashi

  Department Of Applid Chemistry For Resources Tokyo University Of Agriculture And Technology

• NISHIMURA Shigeo

  Department of Applied Chemistry, Faculty of Technology, Tokyo University of Agriculture and Technolo

• SULASWATTY ANNY

  Department of Applid Chemistry for Resources, Tokyo University of Agriculture and Technology

• KITAMURA TAKANORI

  Kuraray Co.,

• KAWAGUCHI TOSHIO

  Kuraray Co.,

• Kitamura Takanori

  Laboratory Of Biochemistry Department Of Biomedical Sciences Graduate School Of Veterinary Medicine

• Nakamura Noriyuki

  Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology:health R

• Nakamura N

  Univ. Tsukuba Tsukuba City Jpn

• Sulaswatty Anny

  Department Of Applid Chemistry For Resources Tokyo University Of Agriculture And Technology

• Kawaguchi Toshio

  Kuraray Co.

• Nishimura Shigeo

  Department Of Applid Chemistry For Resources Tokyo University Of Agriculture And Technology

• NAKAMURA NORIYUKI

  Department of Applid Chemistry for Resources, Tokyo University of Agriculture and Technology

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