Iron Feeding Optimization and Plasmid Stability in Production of Recombinant Bacterial Magnetic Particles by Magnetospirillum magneticum AMB-1 in Fed-Batch Culture
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概要
- 論文の詳細を見る
The production of bacterial magnetic particles (BMPs) by recombinant Magnetospirillum magneticum AMB-1 harboring the plasmid pKML was enhanced in pH-regulated fed-batch culture. The addition of fresh nutrients was feedback-controlled as a function of the pH of the culture. The yield of BMPs was optimized by adjusting the rate of ferric iron addition. Feeding ferric quinate at 15.4 μg/min resulted in a BMP yield of 7.5 mg/l, which is the highest yield so far reported. Expression of a plasmid-encoded fusion protein and segregation of the plasmid during bacterial growth were both stable during fed-batch culture. More than 75% of the cells retained the plasmid for 130 h under antibiotic-free conditions. In addition, the fusion protein permitting the display of a specific protein on the BMP surface was also stably expressed.
- 社団法人日本生物工学会の論文
- 2001-02-25
著者
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Yang Chen
Iab Keio U.
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MATSUNAGA Tadashi
Department of Pediatric Surgery, Chiba University School of Medicine
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TAKEYAMA Haruko
Department of Biotechnology, Tokyo University of Agriculture and Technology
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Matsunaga Tadashi
Department Of Applid Chemistry For Resources Tokyo University Of Agriculture And Technology
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Yang Chendong
Iab Keio U.
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YANG CHENDONG
Department of Biotechnology, Tokyo University of Agriculture and Technology
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Takeyama Haruko
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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