Iron-Regulated Expression and Membrane Localization of the MagA Protein in Magnetospirillum sp. Strain AMB-1.
スポンサーリンク
概要
- 論文の詳細を見る
The magA gene from Magnetospirillum sp. strain AMB-1 is required for the synthesis of bacterial magnetic particles (BMPs). This gene has been cloned, sequenced and found to encode a protein which is homologous to the Escherichia coli potassium efflux membranebinding protein, KefC. By using the firefly luciferase gene (luc) cloned downstream of the magA promoter, the effect of iron on regulation of magA expression was investigated, and transcription of magA was found to be enhanced by low concentrations of iron. Intracellular localization of the MagA protein was studied using magA-luc fusion proteins. The luc gene was cloned downstream of the magA hydrophilic C-terminal domain. Detection of luciferase activity in the cytoplasm, cell membrane, and magnetic particle membrane subcellular fractions confirmed that the MagA fusion protein was localized in the cell membrane. The fusion protein was also detected on the surface of the lipid bilayer covering the magnetic particles. These results suggest that MagA is a membrane-bound protein, the expression of which is enhanced at low iron concentrations.
- 社団法人 日本生化学会の論文
著者
-
Matsunaga Tadashi
Department Of Applid Chemistry For Resources Tokyo University Of Agriculture And Technology
-
Nakamura Chikashi
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology:biomedic
-
Burgess J.
Department Of Biological Sciences Heriot-watt University
-
Kikuchi Tomoko
Department of Biotechnology, Tokyo University of Agriculture and Technology
関連論文
- PJ-546 Exercise Tolerability and QOL in Japanese Heart Transplant Patients(PJ092,Transplantation/LVAD/Cardiovascular Surgery/CABG (M/IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PE-243 Cystatin-C as a Significant Marker of Kidney Dysfunction in Japanese Heart Transplant Patients(PE041,Transplantation/LVAD (M),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PE-242 Serial Assessment of the Characteristics in Cardiac Allograft Vasculopathy by Use of IVUS(PE041,Transplantation/LVAD (M),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- 2P223 培養細胞へのナノニードル挿入に対するフィブロネクチンナノ薄膜の効果(細胞生物的課題(接着,運動,骨格,伝達,膜),第48回日本生物物理学会年会)
- PJ-341 Image Quality of Coronary Computed Tomographic Angiography without Beta-blocker Administration for Heart Transplant Recipients(PJ058,CT/MRI (Coronary/Vascular) 1 (I),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circu
- OE-086 Cardiac MR Measurement of Peak Left Ventricular Filling Rate for Noninvasive Screening of Cardiac Allograft Vasculopathy in Heart Transplant Recipients(OE15,Cardiac Function (Clinical) (M),Oral Presentation (English),The 73rd Annual Scientific Meet
- A Low Invasive Gene Transfer to a Single Cell Using Nanoneedle Operated by AFM
- 2P564 A low invasive and high efficient gene delivery method into a single cell using nanoneedle and AFM(53. Bioengineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 3TA4-08 抗体修飾ナノ針を用いた細胞内繊維状タンパク質の力学検出による細胞選別法の開発(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- Estrogen Secreting Adrenal Adenocarcinoma in an 18-Month-old Boy: Aromatase Activity, Protein Expression, mRNA and Utilization of Gonadal Type Promoter
- 3P-100 AFMを用いたテロメスタチン誘導体とGカルテット構造DNAの結合解析(核酸-相互作用・複合体,第47回日本生物物理学会年会)
- 3P-166 針状材料の細胞挿入成功率に対する骨格タンパク質の寄与(細胞生物学的課題(3),第46回日本生物物理学会年会)
- Development of a Novel Method for Operating Magnetic Particles, Magtration Technology, and Its Use for Automating Nucleic Acid Purification
- Iron-Regulated Expression and Membrane Localization of the MagA Protein in Magentospirillum sp. Strain AMB-1
- β-Carotene production in a novel hydrogen-producing marine photosynthetic bacterium Phodovulum sulfidohpilum expressing the Erythrobacter longus OCh101 crt I and crt Y genes
- Expression of MRP and cMOAT in Childhood Neuroblastomas and Malignant Liver Tumors and Its Relevance to Clinical Behavior
- 1P-322 セルサージェリーによる単一生細胞内酵素活性検出を目指した,固相環境にて機能する新規カスパーゼ3プローブの開発(バイオエンジニアリング,第46回日本生物物理学会年会)
- Optimal Strategy of Preoperative Transcatheter Arterial Chemoembolization for Hepatoblastoma
- P-101 MULTICYSTIC DYSPLASTIC KIDNEY DETECTED BY PRENATAL ULTRASONOGRAPHY : NATURAL HISTORY AND CONSERVATIVE MANAGEMENT
- Strangulated Umbilical Hernia in a Child : Report of a Case
- Digestion of FRET Peptide Layer Using V8 Protease-Immobilized AFM Probe
- High-Sensitivity Detection System for Dioxin Using a Dioxin-Binding Pentapeptide
- MN-P31 Development of a detection method for intracellular mRNA using in situ nanoprobe(Section X Micro/Nano Technology for Analysis and Cell Manipulation)
- 2P563 Detection of mechanical force to unbind complexes between cytoskeletal proteins and the antibodies immobilized on a nanoneedle(53. Bioengineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- IS-17 Shc Family and Trk Family Expression in Neuroblastoma
- IS-13 The search of a novel gene responsible for hirschsprung disease and related neurocristopathies
- 430 Preparation and Mechanical Properties of Carbon Fiber Reinforced AZ91D Magnesium Alloy Composite Fabricated by Squeeze Casting Process
- Recovery of a marine cyanobacterial recombinant product using fish-feed organisms
- 導電性樹脂塗料を用いた電気化学的殺菌および生物付着防止への応用〔英文〕 (バイオエレクトロケミストリ-の新展開)
- フロ-インジェクションシステムによるアルカリホスファタ-ゼ標識IgEを用いたアレルゲンの発光分析〔英文〕 (バイオエレクトロケミストリ-の新展開)
- Use of ferrocene derivative for electrochemical prevention of marine biofouling
- Fully Automated DNA Extraction from Blood Using Magnetic Particles Modified with a Hyperbranched Polyamidoamine Dendrimer(MEDICAL BIOTECHNOLOGY)
- Discrimination between Atlantic and Pacific Subspecies of Northern Bluefin Tuna(Thunnus thynnus)by Magnetic-Capture Hybridization Using Bacterial Magnetic Particles
- N-(Benyloxycarbony)-L-Aspartyl-L-Phenylalanine Methyl Ester Production by Immobilized Nocardia opaca : Thermolysin under Hydrogen High Pressure in a Water-Organic Solvent System
- 219 L-Phenylalanine Methyl Ester Production from Phenylpyruvate Methyl Ester by Immobilized Cells Under Hydrogen High Pressure in Water-Organic Solvent System.
- Two Tandemly Arrayed Transfer-RNA-Derived SINEs of the Medaka (Oryzias latipes)
- Catechol Siderophore Excretion by Magnetotactic Bacterium Magnetospirillum magneticum AMB-1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Iron Feeding Optimization and Plasmid Stability in Production of Recombinant Bacterial Magnetic Particles by Magnetospirillum magneticum AMB-1 in Fed-Batch Culture
- Production of coccolith particle by coccolithophorid alga Emiliania huxleyi
- Construction of an Electrochemical Antibiofouling System for Plate Heat Exchangers
- 319 Continual L-Phenylalanine Production from Phenylpyruvate by Immobilized Cells of Nocardia Opaca Under Hydrogen High Pressure
- 磁性細菌粒子マイクロアレイを用いた一塩基多型解析
- High-Resolution Magnetic Force Microscope Images of a Magnetic Particle Chain Extracted from Magnetic Bacteria AMB-1
- Characterization of nonmagnetic mutant of Magnetospirillum sp. strain AMB-1
- Evaluation of the insertion efficiencies of tapered silicon nanoneedles and invasiveness of diamond nanoneedles in manipulations of living single cells
- Enhanced hydrogen production by a marine photosynthetic bacterium, Rhodobacter marinus, immobilized onto light-diffusing optical fibers
- Tellurite removal by marine photosynthetic bacteria
- Screening of Marine Bacteria for the Production of Microbial Repellents Using a Spectrophotometric Chemotaxis Assay
- Molecular Mechanism of Magnet Formation in Bacteria
- 560 Genetic and Spectroscopic Analysis of Marine Photosynthetic Bacteria
- Surface Attachment Induced Production of Antimicrobial Compounds by Marine Epiphytic Bacteria Using Modified Roller Bottle Cultivation
- Comprehensive evaluation of leukocyte lineage derived from human hematopoietic cells in humanized mice(CELL AND TISSUE ENGINEERING)
- Bioprocess Inensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design
- Comprehensive evaluation of leukocyte lineage derived from human hematopoietic cells in humanized mice
- Cellular Responses to Electrochemical Killing Process by Applying a Constant Potential in Synchronously Cultured Saccharomyces Cerevisiae
- Aggregation and Fibrillation Study of α-synuclein Under Applied Voltage
- Nanoneedle insertion into the cell nucleus does not induce double-strand breaks in chromosomal DNA(CELL AND TISSUE ENGINEERING)
- Fabrication of Genetic Diagnostic Chip using DNA-arrayed TFT Photosensor
- Iron-Regulated Expression and Membrane Localization of the MagA Protein in Magnetospirillum sp. Strain AMB-1.