Nanoneedle insertion into the cell nucleus does not induce double-strand breaks in chromosomal DNA(CELL AND TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
An atomic force microscope probe can be formed into an ultra-sharp cylindrical shape (a nanoneedle) using micro-fabrication techniques such as focused ion beam etching. This nanoneedle can be effectively inserted through the plasma membrane of a living cell to not only access the cytosol, but also to penetrate through the nuclear membrane. This technique shows great potential as a tool for performing intranuclear measurements and manipulations. Repeated insertions of a nanoneedle into a live cell were previously shown not to affect cell viability. However, the effect of nanoneedle insertion on the nucleus and nuclear components is still unknown. DNA is the most crucial component of the nucleus for proper cell function and may be physically damaged by a nanoneedle. To investigate the integrity of DNA following nanoneedle insertion, the occurrence of DNA double-strand breaks (DSBs) was assessed. The results showed that there was no chromosomal DNA damage due to nanoneedle insertion into the nucleus, as indicated by the expression level of γ-H2AX, a molecular marker of DSBs.
- 公益社団法人日本生物工学会の論文
著者
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Nakamura Noriyuki
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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Nakamura Chikashi
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology:biomedic
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Ryu Seunghwan
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
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NAKAMURA NORIYUKI
Department of Applid Chemistry for Resources, Tokyo University of Agriculture and Technology
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Kawamura Ryuzo
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology
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Nakamura Chikashi
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology:Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology
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Naka Ryohei
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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Silberberg Yaron
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology
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