Determination of Insulin Content in Pancreatic β Cell Line MIN6 Cells by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
スポンサーリンク
概要
- 論文の詳細を見る
The insulin content in mouse insulinoma MIN6 cells was determined using matrix-assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry (TOF/MS). A mass spectrum of cellular insulin was obtained with a cell burst of MIN6 by hypotonic water. Signal intensities of intracellular insulin were proportional to the number of MIN6 cells. The present method was applied to the determination of intracellular insulin content of MIN6 before and after glucose stimulation.
- 公益社団法人日本薬学会の論文
- 1998-11-15
著者
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MASUJIMA Tsutomu
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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IKEDA Kayo
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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OZAWA Koichiro
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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TAMURA Atsushi
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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Ikeda Kayo
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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Ozawa Koichiro
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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Ikeda Katsunori
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
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Masujima Tsutomu
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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Masujima Tsutomu
Institue Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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AZUMA Tsuyoshi
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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Masujima T
Hiroshima Univ. School Of Medicine Hiroshima‐shi Jpn
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Masujima T
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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Tamura A
Graduate School Of Medicine Osaka Univ.
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Tamura Atsushi
Institue Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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