VOLTAMMETRIC MICROANALYSIS OF PURINES BY USING IMMOBILIZED ENZYME REACTOR

概要

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The voltammetric microanalysis of naturally occurring purines (uric acid, xanthine, and hypoxanthine) was studied by using a plug flow reactor of immobilized xanthine oxidase. The cyclic voltammetry at a glassy carbon electrode showed highly irreversible and adsorptive characteristics on the electrode surface, and in a mixed solution of purines the anodic peak currents were obscured by the competitive adsorption. Reproducible analytical data were obtained by the oxidative voltammetry using a thin-layer flow cell with samples sufficiently diluted to avoid the adsorption effect. Uric acid in deproteinized plasma was determined at 0.4 V vs. Ag/AgCl at pH 7.7, and the total amount of xanthine and hypoxanthine was estimated under the same condition by using enzymatic oxidation by xanthine oxidase after uric acid in plasma was converted to allantoin by uricase. The c.v. value of the determination at 0.2 nmole was 2.7%, and the detection limit was 10 pmole or less.
社団法人日本分析化学会の論文
1982-03-05