Studies on Variation of Tryptophan Metabolites Level in Rabbit Plasma with Preparation Procedures by High Performance Liquid Chromatography (生体成分の高速液体クロマトグラフィ-)

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Reversed phase high performance liquid chromatography with direct injection of plasma samples was applied to evaluate the variation of plasma tryptophan (Trp) metabolites level with sample preparation procedures. Fresh heparinized rabbit plasma showed the peaks corresponding to serotonin (5-HT), 5-hydroxy indole-3-acetic acid (5HIAA), indole-3-acetic acid (IAA), indole-3-lactic acid (ILA), indole3-propionic acid (IPA), kynurenine (Kyn), kynurenic acid (KA) in addition to tryptophan (Trp). By standing plasma at 37℃, 5-HT trended to decrease about 5%/h, but other metabolites levels were constant for at least 24 h. By standing platelet rich plasma (PRP) for 6 h, 5-HT and 5-HIAA increased. In addition, 5-hydroxy tryptophol (5-OH TrpOL), which was not found as a common metabolite in fresh plasma, was detected. By standing whole blood for 6 h, almost all metabolites except IAA and IPA increased by several times. Serum levels of 5-HIAA, 5-OH TrpOL and especially 5-HT were higher than that of plasma. Consequently, the plasma samples should be prepared just after collecting blood for the analysis of Trp-metabolites level.
社団法人日本分析化学会の論文
1984-07-05